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Molecular and General Genetics MGG

, Volume 135, Issue 3, pp 191–202 | Cite as

Pleiotropic effects of ribosomal protein S4 studied in Escherichia coli mutants

  • M. Olsson
  • L. Isaksson
  • C. G. Kurland
Article

Summary

A temperature sensitive mutant of Escherichia coli was found to have two mutational alterations of its ribosomes: one of these was a streptomycin dependent mutation and the other was a suppressor alteration of S4, with a marked structural change. The altered form of S4 was studied in a strain that was constructed so that this alteration was the only one effecting the structure of the ribosome. Here, it was shown that the mutant form of S4 cause a temperature sensitive defect in the assembly of 30S subunits in vivo which is reflected in the inability of this mutant to properly process ribosomal RNA at the restrictive temperatures. An analysis of both transductants and revertants of this mutant show that the suppression of the streptomycin dependence phenotype, temperature sensitivity, and a defect in RNA processing all have their origin in a single mutational event effecting the structural gene for S4.

Keywords

Escherichia Coli Streptomycin Ribosomal Protein Mutational Event Pleiotropic Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • M. Olsson
    • 1
  • L. Isaksson
    • 1
  • C. G. Kurland
    • 1
  1. 1.Wallenberg LaboratoryUniversity of UppsalaUppsalaSweden

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