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Plant Cell Reports

, Volume 6, Issue 3, pp 256–259 | Cite as

In vitro plantlet formation by organogenesis in E. camaldulensis and by somatic embryogenesis in Eucalyptus citriodora

  • E. M. Muralidharan
  • A. F. Mascarenhas
Article

Abstract

Adventitious shoots were formed through callus on leaf explants of Eucalyptus camaldulensis Dehnh. (River red gum) taken from shoot cultures of mature trees. Callus formed in dark on a medium containing 1 g/l casein hydrolysate, 3 mg/l 1-naphthaleneacetic acid, 0.1 mg/l 6-benzyladenine and 50 g/l sucrose. Shoot initiation occurred in 4 weeks on calli shifted to light on a regeneration medium containing 10% coconut milk, 0.5 mg/l 6-benzyladenine and 20 g/l sucrose. Rooting occured in dark on a liquid medium containing 4 mg/l 1-naphthaleneacetic acid. Zygotic embryos of Eucalyptus citriodora Hook f. (Lemon scented gum) cultured in dark on a medium containing 3 mg/l 1-naphthaleneacetic acid and 50 g/l sucrose formed somatic embryoids which grew to normal plantlets on the same regeneration medium used for organogenesis.

Keywords

Sucrose Somatic Embryogenesis Regeneration Medium Zygotic Embryo Leaf Explants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

6-benzyladenine

CH

Casein hydrolysate

CM

Coconut Milk

NAA

1-naphthaleneacetic acid

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • E. M. Muralidharan
    • 1
  • A. F. Mascarenhas
    • 1
  1. 1.Division of Biochemical SciencesNational Chemical LaboratoryPuneIndia

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