Plant Cell Reports

, Volume 6, Issue 3, pp 256–259 | Cite as

In vitro plantlet formation by organogenesis in E. camaldulensis and by somatic embryogenesis in Eucalyptus citriodora

  • E. M. Muralidharan
  • A. F. Mascarenhas


Adventitious shoots were formed through callus on leaf explants of Eucalyptus camaldulensis Dehnh. (River red gum) taken from shoot cultures of mature trees. Callus formed in dark on a medium containing 1 g/l casein hydrolysate, 3 mg/l 1-naphthaleneacetic acid, 0.1 mg/l 6-benzyladenine and 50 g/l sucrose. Shoot initiation occurred in 4 weeks on calli shifted to light on a regeneration medium containing 10% coconut milk, 0.5 mg/l 6-benzyladenine and 20 g/l sucrose. Rooting occured in dark on a liquid medium containing 4 mg/l 1-naphthaleneacetic acid. Zygotic embryos of Eucalyptus citriodora Hook f. (Lemon scented gum) cultured in dark on a medium containing 3 mg/l 1-naphthaleneacetic acid and 50 g/l sucrose formed somatic embryoids which grew to normal plantlets on the same regeneration medium used for organogenesis.


Sucrose Somatic Embryogenesis Regeneration Medium Zygotic Embryo Leaf Explants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





Casein hydrolysate


Coconut Milk


1-naphthaleneacetic acid


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  1. Aneja S, Atal CK (1969) Curr. Sci. (India) 38:69.Google Scholar
  2. Bennett IJ, McComb JA (1982) Aust. For. Res. 12: 121–127.Google Scholar
  3. Boulay M (1986) In: Proc. 6th Intl. Cong. Plant Tissue and Cell culture, Minneapolis, Minnesota, USA. (In Press).Google Scholar
  4. Diallo N, Duhoux E (1984) J. Plant Physiol. 115: 177–182.Google Scholar
  5. Durand-Cresswell R, Boulay M, Francelet A (1982) In: Bonga JM, Durzan DJ (eds) Tissue Culture in Forestry, Martinus Nijhoff/Dr.W.Junk Publishers, The Hague, pp 150–181.Google Scholar
  6. Gamborg OL, Miller RA, Ojima K (1968): Exp. Cell Res. 50: 151–158.Google Scholar
  7. Gupta PK, Mehta UJ, Mascarenhas AF (1983) Plant Cell Reports 2: 296–299.Google Scholar
  8. Hartney VJ, Barker PK (1980) In: IUFRO Symp. and Workshop on Genetic Improvement and Production of Fast Growing Tree Species, Brazil, 25–30 Aug.Google Scholar
  9. Hu CY, Sussex IM (1971) Phytomorphology 21: 103–107.Google Scholar
  10. Kitahara EH, Caldas LC (1975) Forest Sci. 22 (3): 242–243.Google Scholar
  11. Lakshmi Sita G, (1979) Plant Sci. Lett. 14: 63–68Google Scholar
  12. Litz RE (1985) In: Henke RR, Hughes KW, Constantin MJ, Hollaender A. (eds) Tissue Culture in Forestry and Agriculture. Plenum Press, New York. pp 179–193.Google Scholar
  13. Mascarenhas AF, Gupta PK, Kulkarni VM, Mehta UM, Iyer RS, Khuspe SS, Jagannathan V (1981) In: Rao AN (ed) Proc. COSTED Symp. on Tissue Culture of Economically Important Plants, Singapore pp 175–179.Google Scholar
  14. Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497.Google Scholar
  15. Oka S, Yeung EC, Thorpe TA (1982) New Zealand Journal of Forestry Science 12 (3): 501–509.Google Scholar
  16. Ouyang Q, Pen H, Li Q (1981) Scientia Silvae Sinicae 17: 1–7.Google Scholar
  17. Smith MAL, McCown BH (1983) Plant Sci. Lett. 28: 149–156.Google Scholar
  18. Sondahl MR, Spahlinger DA, Sharp WR (1979) Z. Pflanzenphysiol 94: 101–108.Google Scholar
  19. Tulecke W, McGranahan G (1985) Plant Sci. Lett. 40: 57–63.Google Scholar
  20. Williams EG, Maheswaran G (1986) Ann. Bot. 57: 443–462.Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • E. M. Muralidharan
    • 1
  • A. F. Mascarenhas
    • 1
  1. 1.Division of Biochemical SciencesNational Chemical LaboratoryPuneIndia

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