Molecular and General Genetics MGG

, Volume 181, Issue 2, pp 230–240 | Cite as

Characterization of P1argF derivatives from Escherichia coli K12 transduction

I. IS1 elements flank the argF gene segment
  • Mary K. York
  • Marvin Stodolsky


Specialized transducing derivatives of the temperate bacteriophage P1 (P1std) are selected by transduction into recipients with deletions in the corresponding genes (Stodolsky 1973). When Escherichia coli K12 strains are used as donors in such transduction experiments, P1argF derivatives can be selected. The argF gene is unique to these strains (Glansdorff et al. 1967). Under these experimental conditions P1argF are formed with frequencies 10,000 times greater than other P1std. The majority of the P1argF derivatives that have been analyzed are indistinguishable by cleavage analyses. One such derivative, P1argF5 has been characterized in detail. Heteroduplex analysis against P1, P7, and P1CmO identified an 11 kb insertion of DNA precisely at the naturally occurring IS1 locus of P1. Cleavage analysis with EcoRI, BamHI and PstI confirmed this finding. To further define the argF insertion, a P1Cm13argF derivative was constructed having the IS1 sequences of Cm13 and argF in opposite orientation. Intrastrand annealing of P1Cm13argF5 DNA established that the argF segment is flanked by directly repeated IS1 sequences. The IS1-argF-IS1 segment is desigmated Tn2901. The assignment of the map position of the argF gene within the 11 kb insert of P1argF5 is discussed. The evolutionary significance of this finding and a model for P1argF formation is also presented.


Escherichia Coli Evolutionary Significance Opposite Orientation Temperate Bacteriophage Heteroduplex Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alton N, Vapnek D (1979) Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature 282:864–869Google Scholar
  2. Arber W, Iida S, Jütte H, Caspers P, Meyer J, Hanni C (1978) Rearrangements of genetic material in Escherichia coli as obserbed on the bacteriophage P1 plasmid. Cold Spring Harbor Symp Quant Biol 43:1197–1208Google Scholar
  3. Bächi B, Arber W (1977) Physical mapping of Bg1II, BamHl, EcoRI, HindIII, and PstI restriction fragments of bacteriophage P1 DNA. Mol Gen Genet 153:311–324Google Scholar
  4. Bornhoeft J, Stodolsky M (1980) Plasmid chromosome isolation: An improved batch procedure for large plasmids. Plasmid 4Google Scholar
  5. Campbell A, Berg DE, Botstein D, Lederberg E, Novick R, Starlinger P, Szybalski W (1979): Nomenclature of transposable clements in prokaryotes. Gene 5:197–206Google Scholar
  6. Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acdis. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 21. Academic Press New York, p 413Google Scholar
  7. DeBruijn F, Bukhari AI (1978) Analysis of transposable elements inserted in the genomes of bacteriophages Mu and Pl. Gene 3:315–331Google Scholar
  8. Glansdorff N, Sand G, Verhoef C (1967) The dual genetic control of ornithine transcarbamylase synthesis in Escherichia coli K12. Mutat Res 4:743–751Google Scholar
  9. Hu M, Deonier R (1981) Mapping of IS1 elements flanking the argF gene region on the Escherichia coli K12 chromosome. Mol Gen Genet 181:222–229Google Scholar
  10. Iida S, Arber W (1977) Plaque forming specialized transducing phage P1: Isolation of P1CmSmSu, a precursor of P1Cm. Mol Gen Genet 153:259–269Google Scholar
  11. Iida S, Arber W (1979) Multiple physical differences in the genome structure of functionally related bacteriophages P1 and P7. Mol Gen Genet 173:249–261Google Scholar
  12. Iida S, Arber W (1980) On the role of IS1 in the formation of hybrids between the bacteriophage P1 and the R plasmid NR1. Mol Gen Genet 177:261–270Google Scholar
  13. Iida S, Meyer J, Arber W (1978) The insertion element IS1 is a natural constituent of coliphage P1 DNA. Plasmid 1:357–365Google Scholar
  14. Ikeda H, Tomizawa J (1968) Prophage P1, an extrachromosomal replication unit. Cold Spring Harbor Symp Quant Biol 33:791–798Google Scholar
  15. Johnsrud L (1979) DNA sequence of the transposable element IS1. Mol Gen Genet 169:213–218Google Scholar
  16. Kikuchi A, Gorini L (1975) Similarity of genes argF and arg. Nature 256:621–624Google Scholar
  17. Kikuchi A, Gorini L (1976) Studies of the DNA carrying genes, valS, argI, pryB, and argF by electron microscopy and by site specific endonuclease. J Microscopie Biol Cell 27:1–10Google Scholar
  18. Kondo E, Mitsuhashi S (1964) Drug resistance of enteric bacteria. IV. Active transducing bacteriophage P1 CM produced, by the combination of R factor with bacteriophage P1. J Bacteriol 88:1266–1276Google Scholar
  19. Lee H, Ohtsubo E, Deonier R, Davidson N (1974) Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv + deletion mutants of F14. J Mol Biol 89:585–597Google Scholar
  20. Legrain C, Stalon V, Glansdorff N (1976) Escherichia coli carbomoyltransferase isoenzymes: evolutionary significance and the isolation of λargF and λargI transducing bacteriophages. J Bacteriol 128:35–38Google Scholar
  21. Lennox ES (1955) Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206Google Scholar
  22. Luria S, Adams N, Ting R (1960) Tranduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348–390Google Scholar
  23. MacHattie LA, Jackowski JB (1977) Physical structure and deletion effects of the chloramphenicol resistance element Tn9 in phage lambda. In: Bukhari AI, Shapiro JA, Adhya SL (eds) NDA insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory, New York, p 219Google Scholar
  24. Meyer J, Iida S (1979) Amplification of chloramphenicol resistance transposons carried by phage P1Cm in Escherichia coli. Mol Gen Genet 176:209–219Google Scholar
  25. Moore S, James E (1979) Mapping of restriction sites in the argF gene of Escherichia coli by partial endonuclease digestion of endlabeled DNA. Gene 5:159–175; errata 343–344Google Scholar
  26. Moore S, James E, James P, Fareed G (1978) Isolation, cloning and characterization of argF gene DNA from Escherichia coli K-12. Gene 4:261–278Google Scholar
  27. Novick RP (1974) Bacterial plasmids. In: Laskin AI, Lechevalier HA (eds) Handbook of microbiology, vol IV. CRC Press Cleveland, p 537Google Scholar
  28. Ohtsubo H, Ohtsubo E (1977) Repeated DNA sequences in plasmids, phages, and bacterial chromosomes. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory, New York, p 49Google Scholar
  29. Ohtsubo H, Ohtsubo E (1978) Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci USA 75:615–619Google Scholar
  30. Prentki P, Chandler M, Caro L (1977) Replication of the prophage P1 during the cell cycle in Escherichia coli. Mol Gen Genet 152:71–76Google Scholar
  31. Rae ME, Stodolsky M (1974) Chromosome breakage, fusion and reconstruction during P1dl transduction. Virology 58:32–54Google Scholar
  32. Rosner JL (1972) Formation, induction and curing of bacteriophage P1 lysogens. Virology 48:679–689Google Scholar
  33. Rosner JL (1975) Specialized transduction of pro genes by ∞liphage P1: Structure of a partly diploid P1-pro prophage. Virology 67:42–55Google Scholar
  34. Rosner JL, Guyer MS (1980) Transposition of IS1-λbio-IS1 from a bacteriophage λ derivative carrying the IS1-cat-IS1 transposon (Tn9). Mol Gen Genet 178:111–120Google Scholar
  35. Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes JC, Hutchison III, CA, Slocombe PM, Smith M (1977) Nucleotide sequence of bacteriophage ϕX174 DNA. Nature 265:687–695Google Scholar
  36. Schultz G, Stodolsky M (1976) Integration sites of foreign genes in the chromosome of coliphage P1: A finer resolution. Virology 73:299–302Google Scholar
  37. Scott JR (1968) Genetic studies of bacteriophage P1. Virology 36:564–574Google Scholar
  38. Sens D, Natter W, James E (1977) Evolutionary drift of the argF and argI genes coding for isoenzyme forms of ornithine transcarbamylase in E. coli K12. Cell 10:275–285Google Scholar
  39. Smith HW (1972) Ampicillin resistance in Escherichia coli by phage infection. Nature (London) New Biol 238:205–206Google Scholar
  40. So M, Heffron F, McCarthy B (1979) The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature 277:453–456Google Scholar
  41. Streisinger G, Emrich J, Stahl MM (1967) Chromosome structure in T4. III. Terminal redundancy and length determination. Proc Natl Acad Sci USA 57:292–295Google Scholar
  42. Stodolsky M (1973) Bacteriophage P1 derivatives with bacterial genes: a heterozygote enrichment method for the selection of P1dpro lysogens. Virology 53:471–475Google Scholar
  43. Stodolsky M, Rae M, Mullenbach E (1972) The addition of lac + chromosome fragments to the Escherichia coli proA-proB-lac deletion XIII chromosome. Genetics 70:495–510Google Scholar
  44. Ting RC (1962) The specific gravity of transducing particles of bacteriophage P1. Virology 16:115–121Google Scholar
  45. Yamamoto K, Alberts BM (1970) Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus production. Virology 40:734–744Google Scholar
  46. Yun T, Vapnek D (1977) Electron microscopic analysis of bacteriophages P1, P1Cm and P7: Determination of genome sizes, sequence homology and location of antibiotic resistance determinants. Virology 77:376–385Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Mary K. York
    • 1
  • Marvin Stodolsky
    • 1
  1. 1.Department of MicrobiologyLoyola University, Strich School of MedicineMaywoodUSA

Personalised recommendations