Molecular and General Genetics MGG

, Volume 181, Issue 2, pp 222–229

Mapping of IS1 elements flanking the argF gene region on the Escherichia coli K-12 chromosome

  • Ming Hu
  • Richard C. Deonier
Article

Summary

Two directly-repeated IS1 elments have been mapped on the Escherichia coli K-12 chromosome at positions 23.2 kb and 34.5 kb counterclockwise of the IS3 element α3β3 by using F-prime plasmids (including the F lac- proAB+ plasmid F128) that carry different portions of the bacterial chromosome in the purE to proA region. Mapping was accomplished in part by construction of EcoRI, BamHI, and BglII restriction enzyme cleavage maps. Electron microscope heteroduplex and hybridization studies indicate that the chromosomal region flanked by these IS1 elements is completely homologous to the IS1-argF-IS1 region (Tn2901) on the P1argF5 transducing phage (York and Stodolsky, 1981), which suggests that the argF gene region in the usual E. coli K-12 strains has a transposon-like structure.

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References

  1. Anilionis A, Riley M (1980) Conservation and variation of nucleotide sequences within related genomes: Escherichia coli strains. J Bacteriol 143:355–365Google Scholar
  2. Alton NK, Vapnek D (1979) Nucleotide sequene analysis of the chloramphenicol resistance transposon Tn9. Nature 282:864–869Google Scholar
  3. Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44:1–56Google Scholar
  4. Brachet P, Eisen H, Rambach A (1970) Mutations of coliphage λ affecting the expression of replicative functions O and P. Mol Gen Genet 108:266–276Google Scholar
  5. Calos MP, Johnsrud L, Miller JH (1978) DNA sequences at the integration sites of the insertion element IS1. Cell 13:411–418Google Scholar
  6. Davidson N, Deonier RC, Hu S, Ohtsubo E (1975) Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. X. Deoxyribonucleic acid sequence organization of F and of F-primes, and the sequences involved in Hfr formation. In: Schlessinger D (ed) Microbiology-1974. American Society for Microbiology, Washington, DC p 56Google Scholar
  7. Deonier RC, Mirels L (1977) Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: Involvement of recA. Proc Natl Acad Sci USA 74:3965–3969Google Scholar
  8. Deonier RC, Oh GR, Hu M (1977) Further mapping of IS2 and IS3 in the lac-purE region of the Escherichia coli K-12 genome: Structure of the F-prime ORF203. J Bacteriol 129:1129–1140Google Scholar
  9. Deonier RC, Hadley RG, Hu M (1979) Enumeration and identification of IS3 elements in Escherichia coli strains. J Bacteriol 137:1421–1424Google Scholar
  10. Engel JD, Dodgson JB (1978) Analysis of adult and embryonic chicken globin genes in chromosomal DNA. J Biol Chem 253:8239–8246Google Scholar
  11. Fiandt M, Szybalski W, and Malamy MH (1972) Polar mutations in lac, gal and phage λ consist of a few DNA sequences inserted with either orientation. Mol Gen Genet 119:223–231Google Scholar
  12. Grabeel M, Charlier D, Cunin R, Glansdorff N (1979) Cloning and endonuclease restriction analysis of argF and of the control region of the argECBH biopolar operon in Escherichia coli. Gene 5:207–231Google Scholar
  13. Hadley RG, Deonier RC (1979) Specificity in the formation of type II F′ plasmids. J Bacteriol 139:961–976Google Scholar
  14. Hadley RG, Deonier RC (1980) Specificity in the formation of Δtra F-prime plasmids. J Bacteriol 143:680–692Google Scholar
  15. Hu S, Ohtsubo E, Davidson N (1975) Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: Structure of F13 and related F-primes. J Bacteriol 122:749–763Google Scholar
  16. Iida S, Arber W (1980) On the role of IS1 in the formation of hybrids between the bacteriophage P1 and the R Plasmid NR1. Mol Gen Genet 177:261–270Google Scholar
  17. Kikuchi A, Gorini L (1975) Similarity of genes argF and argI. Nature 256:621–624Google Scholar
  18. Kikuchi A, Gorini L (1976) Studies of the DNA carrying genes, valS, argI, pyrB, and argF by electron microscopy and by site specific endonuclease. J Microscopie Biol Cell 27:1–10Google Scholar
  19. Laskey RA, Mills AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem 56:335–341Google Scholar
  20. Legrain, C, Halleux P, Stalon V, Glansdorff N (1972) The dual genetic control of orinithine carbamoyltransferase in Escherichia coli: a case of bacterial hybrid enzyme. Eur J Biochem 27:93–102Google Scholar
  21. Legrain C, Stalon V, Glansdorff N (1976) Escherichia coli ornithine carbamoyltransferase isoenzymes: evolutionary significance and the isolated of λargF and λargI transducing bacteriophages. J Bacteriol 128:35–38Google Scholar
  22. Ohtsubo E, Deonier RC, Lee H-J, Davidson N (1974) Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli IV. The F sequences in F14. J Mol Biol 89:565–584Google Scholar
  23. Ohtsubo E, and Hsu M-T (1978) Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: Structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attλ-uvrB. J Bacteriol 134:778–794Google Scholar
  24. Parker RC, Seed B (1980) Two-dimensional agarose, gel electrophoresis —“Sea Plaque” agarose dimension. In: Grossman L and Moldave K (eds) Methods in enzymology, vol 65, Pt I. Academic Press, New York, p 358Google Scholar
  25. Polisky B, Greene P, Garfin DE, McCarthy BJ, Goodman HM, Boyer HW (1975) Specificity of substrate recognition by the EcoRI restriction endonuclease. Proc Natl Acad Scid USA 72:3310–3314Google Scholar
  26. Read HA, Sarma SD, Jaskunas SR (1980) Fate of donor insertion sequence ISP1 during transposition. Proc Natl Acad Sci USA 77:2514–2518Google Scholar
  27. Rigby PN, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251Google Scholar
  28. Rosner JL, Guyer MS (1980) Transposition of IS1-λBIO-IS1 from a bacteriophage λ derivative carrying the IS1-cat-IS1 Transposon (Tn9). Mol Gen Genet 178:111–120Google Scholar
  29. Saedler H, Heiss B (1973) Multiple copies of the insertion-DNA, sequences IS1 and IS2 in the chromosome of E. coli, K-12. Mol Gen Genet 122:267–277Google Scholar
  30. Saedler H, Kubai DF, Nomura M, Jaskumas SR (1975) IS1 and IS2 mutations in the ribosomal protein genes of E. coli K-12. Mol Gen Genet 144:85–89Google Scholar
  31. Sharp PA, Hsu M-T, Ohtsubo E, Davidson N (1972) Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I Structure of F-prime factors. J Mol Biol 71:471–497Google Scholar
  32. Smith HO, Birnstiel ML (1976) A simple method for DNA restriction site mapping. Nucl Acid Res 3:2387–2398Google Scholar
  33. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517Google Scholar
  34. Syvanen JM, Roth JR (1972) The structural genes for ornithine transcarbamylase in Salmonella typhimurium and Escherichia coli K-12. J Bacteriol 110:66–70Google Scholar
  35. Thompson R, Hughes SG, Broda P (1974) Plasmid identification using specific endonucleases. Mol Gen Genet 133:141–149Google Scholar
  36. Thuring RWJ, Sanders JPM, Borst P (1975) A freeze-squeneze method for recovering long DNA from agarose gels. Anal Biochem 66:213–220Google Scholar
  37. York MK, Stodolsky M (1981) Characterization of P1argF derivatives from Escherichia coli K-12 transduction: I. IS1 elements flank the argF gene segment. Mol Gen Genet 181:230–240Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Ming Hu
    • 1
  • Richard C. Deonier
    • 2
  1. 1.Department of ChemistryUniversity of Southern CaliforniaLos AngelesUSA
  2. 2.Molecular Biology, Department of Biological SciencesUniversity of Southern CaliforniaLos AngelesUSA

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