Molecular and General Genetics MGG

, Volume 134, Issue 2, pp 181–186 | Cite as

Different effects of rifampicin and streptolydigin on the control of RNA polymerase subunit synthesis in Escherichia coli

  • Ivor P. B. Tittawella
  • Richard S. Hayward


It is known that the drugs rifampicin and streptolydigin both inhibit E. coli RNA polymerase by binding to its β polypeptide subunit. We have previously shown that addition of rifampicin to a strain of E. coli containing both rifampicin-sensitive and-resistant RNA polymerase molecules causes an immediate reduction, in the rates of RNA and total protein synthesis but induces a marked increase in the rate of synthesis of the RNA polymerase subunits β and β′. We now show that addition of streptolydigin to an isogenic strain, containing streptolydigin-sensitive and-resistant RNA polymerase, causes a closely similar reduction in the rates of RNA and total protein synthesis. In this case, however, the rate of β and β′ synthesis declines, closely paralleling the rate of total, protein synthesis. We discuss the implications of these results in relation to the control of RNA polymerase subunit synthesis.


Protein Synthesis Polypeptide Total Protein Rifampicin Similar Reduction 
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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Ivor P. B. Tittawella
    • 1
  • Richard S. Hayward
    • 1
  1. 1.MRC Molecular Genetics UnitUniversity of EdinburghScotland

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