Theoretical and Applied Genetics

, Volume 77, Issue 6, pp 768–776 | Cite as

Distribution of restriction site polymorphism within the chloroplast genome of the genus Glycine, subgenus Soja

  • P. S. Close
  • R. C. Shoemaker
  • P. Keim


Restriction fragment length polymorphisms (RFLPs) have been used to detect intragenic sequence diversity in Glycine subgenus soja chloroplast DNA. The distribution of these RFLPs allow Glycine max and G. soja accessions to be grouped according to cytoplasmic genetic relatedness. DNA clones from mung bean chloroplast DNA were used to locate the RFLPs to specific regions of the chloroplast genome. In the course of the experiments, several previously unobserved RFLPs were also identified. At least six molecular changes were detected, including both restriction site loss or gain and insertion/deletion events. Three of the fragment polymorphisms detected are due to changes in the juncture region between one inverted repeat region and the large single-copy region. Probes detecting polymorphisms in three representative soybean genotypes were used to screen additional cultivars and Plant Introductions. The distribution of RFLP patterns in these accessions were consistent with the patterns of previously described cytoplasmic groupings, with the exception of one accession, which formed a new plastome group.

Key words

Chloroplast DNA RFLP Soybean 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • P. S. Close
    • 1
  • R. C. Shoemaker
    • 2
  • P. Keim
    • 3
  1. 1.Department of GeneticsIowa State UniversityAmesUSA
  2. 2.USDA-ARS, Departments of Agronomy and GeneticsIowa State UniversityAmesUSA
  3. 3.Department of BiologyNorthern Arizona UniversityFlagstaffUSA

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