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Molecular and General Genetics MGG

, Volume 172, Issue 2, pp 185–192 | Cite as

Evidence for mutations in the structural gene for homocitrate synthase in Saccharomycopsis lipolytica

  • Claude Gaillardin
  • Henri Heslot
Article
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Summary

Eight strains devoid of homocitrate synthase activity were found among lysine requiring mutants of the yeast Saccharomycopsis lipolytica. Genetic analysis of these strains showed that they were all affected at the same locus LYS 1. Three lines of evidence suggest that this locus defines a structural gene for homocitrate synthase. First, the mutations show various degrees of intragenic complementation; it could be shown in some cases that the hybrid enzyme formed in vivo displayed modified properties in vitro. Second, reversion of some of these mutations can result in a modified enzyme (desensitized). Third, a feedback mutant of homocitrate synthase was directly isolated from the wild type strain, and shown to carry a single mutation at or near LYS 1.

We also present here the first attempts at genetic fine mapping in Saccharomycopsis lipolytica.

Keywords

Enzyme Lysine Genetic Analysis Structural Gene Type Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

lys

lysine

arg

arginine

ade

adenine

ura

uracile

TDL

4,5-transdehydrolysine

Sm

Saccharomycopsis

KR

kilorads

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Claude Gaillardin
    • 1
  • Henri Heslot
    • 1
  1. 1.Laboratoire de génétiqueInstitut National AgronomiqueParisFrance

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