Advertisement

Applied Microbiology and Biotechnology

, Volume 28, Issue 4–5, pp 340–344 | Cite as

Influence of pH, lactose and lactic acid on the growth of Streptococcus cremoris: a kinetic study

  • Bernard Bibal
  • Gérard Goma
  • Yves Vayssier
  • Alain Pareilleux
Biotechnology

Summary

The effect of various culture conditions on growth kinetics of an homofermentative strain of the lactic acid bacterium Streptococcus cremoris were investigated in batch cultures, in order to facilitate the production of this organism as a starter culture for the dairy industry. An optimal pH range of 6.3–6.9 was found and a lactose concentration of 37 g·l-1 was shown to be sufficient to cover the energetic demand for biomass formation, using the recommended medium. The study of the effect of lactic acid concentration on growth kinetics revealed that the end-product was not the sole factor affecting growth. The strain was characterized for its tolerance towards lactic acid and a critical concentration of 70 g·l-1 demonstrated. With the product yield of 0.9 g·g-1 at non-lactose limiting conditions the lactic acid concentration of 33 g·l-1 could not explain the low growth rates obtained, implicating a nutritional limitation.

Keywords

Biomass Lactic Acid Lactose Lactic Acid Bacterium Product Yield 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Symbols

tf

fermentation duration (h)

X

Biomass concentration (g·l-1)

Xm

maximum biomass concentration (g·l-1)

S

lactose concentration (g·l-1)

Sr

residual lactose concentration (g·l-1)

P

produced lactic acid concentration (g·l-1)

Pa

added lactic acid concentration (g·l-1)

Pc

critical lactic acid concentration (g·l-1)

μ

specific growth rate (h-1)

μmax

maximum specific growth rate (h-1)

Rx/S

biomass yield (g·g-1) calculated when μ=0

RP/S

product yield (g·g-1)

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blickstad E, Molin G (1981) Growth and lactic acid production of Pediococcus pentosaceus at different gas environments, temperatures, pH values and nitrite concentrations. Eur J Appl Microbiol Biotechnol 13:170–174Google Scholar
  2. Brink BT, Konings WN (1982) Electrochemical proton gradient and lactate concentration gradient in Streptococcus cremoris cells grown in batch culture. J Bacteriol 152:682–686Google Scholar
  3. Brink BT, Otto R, Hansen UP, Konings WN (1985) Energy recycling by lactate efflux in growing and nongrowing cells of Streptococcus cremoris. J Bacteriol 162:383–390Google Scholar
  4. Famelart MH, Kobilinsky A, Bouillanne C, Desmazeaud MJ (1987) Influence of temperature, pH and dissolved oxygen on growth of Brevibacterium linens in a fermentor. Eur J Appl Microbiol Biotechnol 25:442–448Google Scholar
  5. Friedman MR, Gaden EL (1970) Growth and acid production by Lactobacillus delbrueckii in a dialysis culture system. Biotechnol Bioeng 12:961–974Google Scholar
  6. Keller AK, Gerhardt P (1975) Continuous lactic acid fermentation of whey to produce a ruminant feed supplement high in crude protein. Biotechnol Bioeng 17:997–1018Google Scholar
  7. Luedecking R, Piret EL (1959) A kinetic study of the lactic acid fermentation. J Biochem Microbiol Tech Eng 1:393–412Google Scholar
  8. Otto R, Brink BT, Veldkamp H, Konings WN (1983) The relation between growth rate and electrochemical proton gradient of Streptococcus cremoris. FEMS Microbiol Lett 16:69–74Google Scholar
  9. Rhee SK, Pack MY (1980) Effect of environmental pH on fermentation balance of Lactobacillus bulgaricus. J Bacteriol 144:217–221Google Scholar
  10. Rogers PL, Bramall L, Mac Donald IJ (1978) Kinetic analysis of batch and continuous culture of Streptococcus cremoris HP. Can J Microbiol 24:372–380Google Scholar
  11. Roy D, Goulet J, Le Duy A (1986) Batch fermentation of whey ultrafiltrate by Lactobacillus helveticus for lactic acid production. Eur J Appl Microbiol Biotechnol 24:206–213Google Scholar
  12. Tsao GT, Hanson TP (1975) Extended monod equation for batch cultures with multiple exponential phases. Biotechnol Bioeng 17:1591–1598Google Scholar
  13. Vick Roy TB (1983) Lactic acid production in membrane fermentors recycle by Lactobacillus delbrueckii. Ph D Thesis, Berkeley University California, pp 61–64Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Bernard Bibal
    • 1
  • Gérard Goma
    • 1
  • Yves Vayssier
    • 2
  • Alain Pareilleux
    • 1
  1. 1.Centre de Transfert en Biotechnologie Microbiologie Département de Génie Biochimique et Alimentaire, UA-CNRS-No 544Institut National des Sciences AppliquéesToulouse CedexFrance
  2. 2.Lacto-Labo-RPDangé St RomainFrance

Personalised recommendations