, Volume 97, Issue 3, pp 213–220

Distribution of insulin binding sites on Leydig cells of rat testes using insulin-coated gold particles

  • M. Kimura
  • A. Lukinius
  • J. L. E. Ericsson
  • L. Grimelius


The distribution of insulin binding sites in Leydig cells dispersed with collagenase from rat testes was studied using insulin-coated gold particles as an electron opaque ligand. Using electron microscope is convenient to distinguish Leydig cells among a variety of cells in crude preparations by their ultrastructural characteristics. Leydig cells were shown to possess insulin-binding sites on their plasma membranes. Initial binding sites of insulin were located to the microvillous surfaces. Following binding, receptor-ligand complexes seemed to move to the intermicrovillous plasma membrane, then to be internalized. Two modes of the internalization were confirmed. Most of the receptor-ligand complexes on Leydig cells appeared to be internalized via large, uncoated plasma membrane invaginations, while the remainder became internalized via small pits into vesicles. The receptor-ligand complexes were subsequently transferred to large subsurface vacuoles with electron-lucent lumens believed to correspond to endosomes. The reason why IGCs on the postendosomal pathway moving toward lysosomes was also discussed.


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • M. Kimura
    • 1
  • A. Lukinius
    • 2
  • J. L. E. Ericsson
    • 2
  • L. Grimelius
    • 2
  1. 1.Department of AnatomyAichi Medical UniversityAichiJapan
  2. 2.Department of Pathology, University of UppsalaUniversity HospitalUppsalaSweden

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