Molecular and General Genetics MGG

, Volume 171, Issue 3, pp 229–238 | Cite as

Mitochondrial DNA from Podospora anserina

I. Isolation and characterization
  • Donald J. Cummings
  • Léon Belcour
  • Claude Grandchamp
Article
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Summary

Mitochondrial (Mt) DNA from Podospora anserina was isolated and characterized with respect to density in CsCl, contour length and endonuclease restriction enzymes. The density of Mt DNA for four races examined was 1.694 g/cm3, compared with 1.712 g/cm3 for nuclear DNA. Extraction in the presence of a nuclease inhibitor, aurintricarboxylic acid and isolation in DAPI CsCl gradients allowed us to isolate high molecular weight DNA. Mt DNA isolated by total DNA extraction contained ca. 1% of circular molecules, 31 μm in contour length; Mt DNA isolated from purified mitochondria contained 2–4% of these 31 μm circles. Analysis with Eco RI restriction endonuclease revealed that each of the four races examined, s, A, T and E had a characteristic fragment pattern. Races s and A Mt DNA differed by only one fragment after Eco RI enzymatic digestion; similarly, these two DNA differed by only one or two fragments after Hae III digestion.

Keywords

DAPI CsCl Contour Length Characteristic Fragment Fragment Pattern 
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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Donald J. Cummings
    • 1
  • Léon Belcour
    • 1
  • Claude Grandchamp
    • 1
  1. 1.Centre de Génétique Moléculaire du CNRSGif-sur-YvetteFrance

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