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Molecular and General Genetics MGG

, Volume 160, Issue 2, pp 225–229 | Cite as

A mutation leading to the total lack of nitrite reductase activity in Escherichia coli K 12

  • M. Chippaux
  • D. Giudici
  • A. Abou-Jaoudé
  • F. Casse
  • M. C. Pascal
Article

Summary

Mutants of E. coli, completely devoid of nitrite reductase activity with glucose or formate as donor were studied. Biochemical analysis indicates that they are simultaneously affected in nitrate reductase, nitrite reductase, fumarate reductase and hydrogenase activities as well as in cytochrome c552 biosynthesis. The use of an antiserum specific for nitrate reductase shows that the nitrate reductase protein is probably missing. A single mutation is responsible for this phenotype: the gene affected, nir R, is located close to tyr R i.e. at 29 min on the chromosomal map.

Keywords

Glucose Nitrate Nitrite Biochemical Analysis Nitrate Reductase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BV

Benzyl-Viologen

NTG

N-methyl-N-nitro-N-nitrosoguanidine

NR

nitrate reductase

NIR

nitrite reductase

FR

fumarate reductase

HYD

hydrogenase

CYT c552

cytochrome c552

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References

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • M. Chippaux
    • 1
  • D. Giudici
    • 1
  • A. Abou-Jaoudé
    • 1
  • F. Casse
    • 1
  • M. C. Pascal
    • 1
  1. 1.Laboratoire de Chimie Bactérienne C.N.R.S.Marseille Cedex 2France

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