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Molecular and General Genetics MGG

, Volume 180, Issue 1, pp 35–46 | Cite as

The hsd (host specificity) genes of E. coli K12

  • Bela Sain
  • Noreen E. Murray
Article

Summary

The hsd genes of E. coli K12 have been cloned in phage λ by a combination of in vitro and in vivo techniques. Three genes, whose products are required for K-specific restriction and modification, have been identified by complementation tests as hsdR, M, and S. The order of these closely linked genes was established as R, M, S by analysis of the DNA of genetically characterised deletion derivatives of λhsd phages. The three genes are transcribed in the same direction but not necessarily as a single operon. Genetic evidence identifies two promoters, one from which transcription of hsdM and S is initiated and a second for the hsdR gene.

The hsdR gene codes for a polypeptide of molecular weight ∼130000; hsdM for one of 62–65000 and the hsdS gene was associated with two polypeptides of approximately 50000. Circumstantial evidence suggest that one of these two polypeptides may be a degradation, or processed, derivative of the other. The hsdS polypeptide of E. coli B has a slightly higher mobility in an SDS-polyacrylamide gel than does that of E. coli K 12.

A probe comprising most of the hsdR gene and all of the hsdM and S genes of E. coli K 12 shares extensive homology with the DNA of E. coli B but none with that of E. coli C.

Keywords

Molecular Weight Polypeptide Circumstantial Evidence High Mobility Host Specificity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Bela Sain
    • 1
  • Noreen E. Murray
    • 1
  1. 1.Department of Molecular BiologyUniversity of EdinburghEdinburghScotland

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