Molecular and General Genetics MGG

, Volume 120, Issue 2, pp 181–190 | Cite as

The origin of DNA in transducing particles in P22-mutants with increased transduction-frequencies (HT-mutants)

  • Horst Schmieger
  • Horst Backhaus


The physical properties of the transducing DNA of HT-phage mutants are described. It could be shown that the increased frequency of transducing particles is not due to replication of such structures formed at a normal rate, but rather to an increased rate of their formation. Further it turned out that density and the sedimentation coefficient of transducing DNA of HT-mutants is comparable to transducing DNA of wild type phages. It could be confirmed that there is also a contribution of about 10% newly synthesized DNA to the bacterial fragment. Therefore it is concluded that the mechanism which leads to transducing particles in HT-mutants is the same as in wild type phages.


Sedimentation Normal Rate Type Phage Sedimentation Coefficient Wild Type Phage 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Horst Schmieger
    • 1
  • Horst Backhaus
    • 1
  1. 1.Abt. Molekulare GenetikInstitut für Mikrobiologie der Gesellschaft für Strahlen- und UmweltforschungGöttingenGermany

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