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Molecular and General Genetics MGG

, Volume 120, Issue 2, pp 181–190 | Cite as

The origin of DNA in transducing particles in P22-mutants with increased transduction-frequencies (HT-mutants)

  • Horst Schmieger
  • Horst Backhaus
Article

Summary

The physical properties of the transducing DNA of HT-phage mutants are described. It could be shown that the increased frequency of transducing particles is not due to replication of such structures formed at a normal rate, but rather to an increased rate of their formation. Further it turned out that density and the sedimentation coefficient of transducing DNA of HT-mutants is comparable to transducing DNA of wild type phages. It could be confirmed that there is also a contribution of about 10% newly synthesized DNA to the bacterial fragment. Therefore it is concluded that the mechanism which leads to transducing particles in HT-mutants is the same as in wild type phages.

Keywords

Sedimentation Normal Rate Type Phage Sedimentation Coefficient Wild Type Phage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Horst Schmieger
    • 1
  • Horst Backhaus
    • 1
  1. 1.Abt. Molekulare GenetikInstitut für Mikrobiologie der Gesellschaft für Strahlen- und UmweltforschungGöttingenGermany

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