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Molecular and General Genetics MGG

, Volume 120, Issue 2, pp 107–114 | Cite as

Temperature-sensitive mutations in Drosophila melanogaster

XIV. A selection of immobile adults
  • Thomas A. Grigliatti
  • Linda Hall
  • Raja Rosenbluth
  • David T. Suzuki
Article

Summary

Improved methods for rearing and screening large numbers of flies permitted the recovery of 10 mutations exhibiting a reversible temperature-dependent adult paralysis among 1.1×106 flies tested. Of the 10 mutations, two were allelic to parats,two were alleles in a new locus, stoned (stn), and six fell into a third area, the shibire (shi) locus. Several of the shi alleles cause embryonic, larval and adult paralysis at 29° C as well as structural anomalies of various tissues. In addition to the ts mutations, several non-conditional mutations affecting adult movement were recovered.

Keywords

Improve Method Structural Anomaly Adult Movement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baillie, D., Suzuki, D. T., Tarasoff, M.: Temperature-sensitive mutations in Drosophila melanogaster. II. Frequency among second chromosome recessive lethals induced by ethyl methanesulfonate. Canad. J. Genet. Cytol. 10, 412–420 (1968).Google Scholar
  2. Grigliatti, T., Suzuki, D. T.: Temperature-sensitive mutations in Drosophila melanogaster. V. A mutation affecting concentrations of pteridines. Proc. nat. Acad. Sci. (Wash.) 67, 1101–1108 (1970).Google Scholar
  3. Grigliatti, T., Suzuki, D. T., Williamson, R.: Temperature-sensitive mutations in Drosophila melanogaster. X. Developmental analysis of the paralytic mutation, para ts.Develop. Biol. 28, 352–371 (1972)Google Scholar
  4. Hotta, Y., Benzer, S: Abnormal electroretinograms in visual mutants of Drosophila. Nature (Lond.) 222, 354–356 (1969).Google Scholar
  5. Ikeda, K., Kaplan, W. D.: Patterned neural activity of a mutant Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.). 66, 765–772 (1970).Google Scholar
  6. Judd, B. H., Shen, M. W., Kaufman T. C.: The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics 71, 139–156 (1972).Google Scholar
  7. Konopka, R. J., Benzer, S.: Clock mutants of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 68, 2112–2116 (1971).Google Scholar
  8. Lefevre G., Jr.: A new symbol to distiguish insertional from reciprocal translocations. Drosophila Inform. Serv. 48, 158 (1972).Google Scholar
  9. Lewis, E. B., Bacher, F.: Method of feeding ethyl methanesulfonate (EMS) to Drosophila males. Drosophila Inform. Serv. 43, 193 (1968).Google Scholar
  10. Lindsley, D. L., Grell, E. H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. 627 (1968).Google Scholar
  11. Pak, W. L., Grossfield, J., White, N. V.: Nonphototactic mutants in a study of vision of Drosophila. Nature (Lond.) 222, 351–354 (1969).Google Scholar
  12. Poodry, C., Hall, L., Suzuki, D. T.: Developmental properties of shibire ts1, a pleiotropic mutation producing larval and adult paralysis. Develop. Biol., in press (1973).Google Scholar
  13. Suzuki, D. T.: Temperature-sensitive mutations in Drosophila melanogaster. Science 170, 695–706 (1970).Google Scholar
  14. Suzuki, D. T., Grigliatti, T., Williamson, R.: Temperature-sensitive mutations in Drosophila melanogaster. VII. A mutation (para ts)causing reversible adult paralysis. Proc. nat. Acad. Sci. (Wash.) 68, 890–893 (1971).Google Scholar
  15. Suzuki, D. T., Piternick, L. K., Hayashi, S., Tarasoff, M., Baillie, D., Erasmus, U.: Temperature-sensitive mutations in Drosophila melanogaster. I. Relative frequencies among γ-ray and chemically induced sex-linked lethals and semi-lethals. Proc. nat. Acad. Sci. (Wash.) 58, 907–912 (1967).Google Scholar
  16. Williamson, R.: A screening device for separation of immobilized adults from normal flies. Drosophila Inform. Serv. 46, 148–149 (1971).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Thomas A. Grigliatti
    • 1
    • 2
  • Linda Hall
    • 1
  • Raja Rosenbluth
    • 1
  • David T. Suzuki
    • 1
  1. 1.Department of ZoologyThe University of British ColumbiaVancouverCanada
  2. 2.Biochemistry DepartmentUniversity of British ColumbiaVancouver 8Canada

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