Molecular and General Genetics MGG

, Volume 178, Issue 1, pp 237–240 | Cite as

Length determination of the terminal redundant regions in the DNA of phage T7

  • Brigitte Dreiseikelmann
  • Ursula Steger
  • Wilfried Wackernagel
Short Communication


The length of the terminal redundant regions in T7 DNA has been determined by two methods. One involved the specific labeling and isolation of the redundant DNA fragment and determination of the molecular weight by polyacrylamide gel electrophoresis. A value of 150±10 nucleotide pairs was obtained. The other determination based on a correlation of the melting temperature of the redundant region to that of whole T7 DNA confirmed the result obtained by the first method.


Molecular Weight Nucleotide Electrophoresis Polyacrylamide Melting Temperature 
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  1. Britten, R.J., Graham, D.E., Neufeld, B.R.: Analysis of repeating DNA sequences by reassociation. In: Methods in enzymologie XXIX, part E (L. Grossman and K. Moldave, eds.), pp. 363–418. New York-London: Academic Press 1974Google Scholar
  2. Burd, J.F., Wartell, R.M., Dodgson, J.B., Wells, R.D.: Transmission of stability (telestability) in deoxyribonucleic acid. J. Biol. Chem. 250, 5109–5113 (1975)Google Scholar
  3. Crothers, D.M., Kallenbach, N.R., Zimm, B.H.: The melting transition of low-molecular-weight DNA: theory and experiment. J. Mol. Biol. 11, 802–820 (1965)Google Scholar
  4. Dreiseikelmann, B., Eichenlaub, R., Wackernagel, W.: The effect of differential methylation by Escherichia coli of plasmid DNA and phage T7 and λ DNA on the cleavage by restriction endonuclease MboI from Moraxella bovis. Biochim. Biophys. Acta 562, 418–428 (1979)Google Scholar
  5. Dreiseikelmann, B., Wackernagel, W.: The terminal redundant regions of bacteriophage T7 DNA: their necessity for phage production studies by the infectivity of T7 DNA after modification by various exonucleases. Mol. Gen. Genet. 159, 321–328 (1978)Google Scholar
  6. Ehrlich, S.D., Sagaramella, V., Lederberg, J.: Transfection of restrictionless Escherichia coli by bacteriophage T7 DNA: Effect of in vitro erosion of DNA by λ exonuclease. J. Mol. Biol. 105, 603–609 (1976)Google Scholar
  7. Glynn, I.M., Chappel, J.B.: A simple method for the preparation of 32P-labeled adenosine triphosphate of high specific activity. Biochem. J. 90, 147–149 (1964)Google Scholar
  8. Hausmann, R.: Bacteriophage T7 genetics. Curr. Top. Microbiol. Immunol. 75, 77–110 (1976)Google Scholar
  9. Langman, L., Paetkau, V., Scraba, D., Miller, R.C., Jr., Roeder, G.S., Sadowski, P.D.: The structure and maturation of intermediates in bacteriophage T7 DNA replication. Can. J. Biochem. 56, 508–516 (1978)Google Scholar
  10. Little, J.W., Lehman, I.R., Kaiser, A.D.: An exonuclease induced by bacteriophage λ, I. Preparation of the crystalline enzyme. J. Biol. Chem. 242, 672–678 (1967)Google Scholar
  11. Ludwig, A.R., Summers, W.C.: A restriction fragment analysis of the T7 left early region. Virology 68, 360–373 (1975)Google Scholar
  12. McDonell, S.W., Simon, M.N., Studier, F.W.: Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J. Mol. Biol. 110, 119–146 (1977)Google Scholar
  13. Richardson, C.C.: Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected E. coli. Proc. Natl. Acad. Sci. U.S.A. 54, 158–165 (1965)Google Scholar
  14. Richardson, C.C., Lehman, J.R., Kornberg, A.: A DNA phosphatase-exonuclease from E. coli. II. Characterization of the exonuclease activity. J. Biol. Chem. 239, 251–258 (1964)Google Scholar
  15. Ritchie, D.A., Thomas, C.A., McHattie, L.A., Wensink, P.C.: Terminal repetition in non-permutated T3 and T7 bacteriophage DNA molecules. J. Mol. Biol. 23, 365–376 (1967)Google Scholar
  16. Seroka, K., Wackernagel, W.: In vivo effects of recBC DNase, exonuclease I, and DNA polymerase of Escherichia coli on the infectivity of native and single-stranded DNA of bacteriophage T7. J. Virol. 21, 906–912 (1977)Google Scholar
  17. Studier, F.W.: Bacteriophage T7. Science 176, 367–376 (1972)Google Scholar
  18. Sussenbach, J.S., Monfoort, C.H., Schiphof, R., Stobberingh, E.E.: A restriction endonuclease from Staphylococcus aureus. Nucleic Acids Res. 3, 3193–3202 (1976)Google Scholar
  19. Sutcliffe, J.G.: pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 5, 2721–2728 (1978)Google Scholar
  20. Vogt, V.M.: Purification and further properties of single-strandspecific nuclease from Aspergillus oryzae. Eur. J. Biochem. 33, 192–200 (1973)Google Scholar
  21. Watson, J.D.: Origin of concatemeric T7 DNA. Nature 239, 197–201 (1972)Google Scholar
  22. Wilson, D.A., Thomas, C.A., Jr.: Palindromes in chromosomes. J. Mol. Biol. 84, 115–144 (1974)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Brigitte Dreiseikelmann
    • 1
  • Ursula Steger
    • 1
  • Wilfried Wackernagel
    • 1
  1. 1.Lehrstuhl Biologie der MikroorganismenRuhr-UniversitätBochumFederal Republic of Germany

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