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Molecular and General Genetics MGG

, Volume 178, Issue 1, pp 237–240 | Cite as

Length determination of the terminal redundant regions in the DNA of phage T7

  • Brigitte Dreiseikelmann
  • Ursula Steger
  • Wilfried Wackernagel
Short Communication

Summary

The length of the terminal redundant regions in T7 DNA has been determined by two methods. One involved the specific labeling and isolation of the redundant DNA fragment and determination of the molecular weight by polyacrylamide gel electrophoresis. A value of 150±10 nucleotide pairs was obtained. The other determination based on a correlation of the melting temperature of the redundant region to that of whole T7 DNA confirmed the result obtained by the first method.

Keywords

Molecular Weight Nucleotide Electrophoresis Polyacrylamide Melting Temperature 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Brigitte Dreiseikelmann
    • 1
  • Ursula Steger
    • 1
  • Wilfried Wackernagel
    • 1
  1. 1.Lehrstuhl Biologie der MikroorganismenRuhr-UniversitätBochumFederal Republic of Germany

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