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Molecular and General Genetics MGG

, Volume 120, Issue 3, pp 211–226 | Cite as

A protein produced by male strains of Escherichia coli K-12 which increases the yield of recombinants in conjugation: Its nature and mode of action

  • D. M. Goldfarb
  • G. I. Goldberg
  • L. S. Chernin
  • L. A. Gukova
  • I. D. Avdienko
  • B. N. Kuznetsova
  • I. Ch. Kushner
Article

Summary

A protein present in filtrates of E. coli K-12 male strains is responsible for their ability to increase the yield of recombinants in conjugation and to inhibit nitrogen mustard after-effect (NMAE) in F- cells. The protein, designated “recombination-stimulating factor” (RSF), was purified 200–300 times from HfrC filtrate. Two Rsf- mutants of strain HfrC were isolated which fail to produce RSF; these mutations affect an episomal gene. RSF action involves the attachment of RSF to the F- cell surface and requires the integrity of the RSF-cell complex. Some step of recombination after the transfer of the donor chromosomal fragment into the recipient is affected by RSF.

Keywords

Nitrogen Recombination Cell Surface Chromosomal Fragment Nitrogen Mustard 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • D. M. Goldfarb
    • 1
  • G. I. Goldberg
    • 1
  • L. S. Chernin
    • 1
  • L. A. Gukova
    • 1
  • I. D. Avdienko
    • 1
  • B. N. Kuznetsova
    • 1
  • I. Ch. Kushner
    • 1
  1. 1.Institute of General GeneticsUSSR Academy of SciencesMoscowUSSR

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