Molecular and General Genetics MGG

, Volume 121, Issue 3, pp 259–269 | Cite as

Mapping of chlorate-resistant mutants of Citrobacter freundii by deletion and complementation analysis

  • J. de Graaff
  • Wilma Barendsen
  • A. H. Stouthamer
Article
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Summary

From Citrobacter freundii mutants have been isolated, with deletions extending chl genes. 53% of these mutants mapped in the gal-bio region of the chromosome. Genetic mapping by three methods—deletion analysis, linkage analysis in crosses with C. freundii Hfr donors and complementation with Escherichia coli F′ factors—establishes the gene order: chl H-gal-chl D-hut-bio-uvr B-chl A-chl I-chl E In an other segment a gene order ilv-chl-pdx was found. Furthermore chl loci were found adjacent to 7 different chromosomal markers.

C. freundii can form nitrate reductase A, thiosulfate reductase, tetrathionate reductase and formic dehydrogenase. These enzymes are not formed in most of the chlorate-resistant mutants. In some of these mutants the enzyme activities can be restored by complementation with F′ factors of E. coli.

Keywords

Enzyme Nitrate Enzyme Activity Genetic Mapping Linkage Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • J. de Graaff
    • 1
    • 2
  • Wilma Barendsen
    • 1
    • 2
  • A. H. Stouthamer
    • 1
    • 2
  1. 1.Laboratory for Medical MicrobiologyFree UniversityAmsterdamThe Netherlands
  2. 2.Biological Laboratory, Microbiology DepartmentFree UniversityAmsterdamThe Netherlands

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