Molecular and General Genetics MGG

, Volume 143, Issue 2, pp 223–232

Isolation of Rec mutants from an F-prime merodiploid strain of Escherichia coli K-12

  • K. A. Stacey
  • Robert G. Lloyd


This paper describes a method of screening mutagenised populations of an E. coli galAgalBF-prime merodiploid for mutants defective in recombination. The method relies on scoring colonies on Eosin-Methylene Blue agar that have fewer than normal numbers of Gal+ papillae. With a suitable choice of galmutations most of the papillae arise by recombination and some of those colonies with less than normal numbers prove to be defective in some aspect of recombination or DNA repair. In addition to strains carrying mutations that can be ascribed to known loci, several novel mutant phenotypes were identified.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adams, M.H.: Bacteriophages. New York: Interscience Publishers Inc. 1959Google Scholar
  2. Bachmann, B.J.: Pedigrees of some mutant strains of Escherichia coli K-12. Bact. Rev 36, 525–557 (1972)Google Scholar
  3. Birge, E.A., Low, K.B.: The detection of transcribable recombination products following conjugation in rec +, recB and recC strains of Escherichia coli K-12. J. molec. Biol. 83, 447–457 (1974)Google Scholar
  4. Cerda-Olmeda, E., Hanawalt, P.D.: Repair of DNA damaged by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli. Mutation Res. 4, 369–371 (1967)Google Scholar
  5. Clark, A.J.: Recombination deficient mutants of E. coli and other bacteria. Ann. Rev. Genet. 7, 67–86 (1973)Google Scholar
  6. Clark, A.J., Margulies, A.D.: Isolation and characterisation of recombination deficient mutants of Escherichia coli K-12. Proc. nat. Acad. Sci. (Wash.) 53, 451–459 (1965)Google Scholar
  7. De Lucia, P., Cairns, J.: Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature (Lond.) 224, 1164–1166 (1969)Google Scholar
  8. Evenchik, Z., Stacey, K.A., Hayes, W.: Ultraviolet induction of chromosome transfer by autonomous sex factors in Escherichia coli. J. gen. Microbiol. 56, 1–14 (1969)Google Scholar
  9. Goldmark, P.J., Linn, S.: Purification and properties of the recBC DNase of Escherichia coli K-12. J. biol. Chem. 247, 1849–1860 (1972)Google Scholar
  10. Gross, G., Gross, M.: Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase Nature (Lond.) 224, 1166–1169 (1969)Google Scholar
  11. Hall, J.D., Howard-Flanders, P.: Recombinant F′ factors from Escherichia coli K-12 strains carrying recB or recC. J. Bact. 110, 578–584 (1972)Google Scholar
  12. Hirota, Y.: The effect of acridine dyes on mating type factors in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 46, 57–64 (1960)Google Scholar
  13. Howard-Flanders, P.: Genes that control DNA repair and genetic recombination in Escherichia coli. Adv. viol. med. Physics 1, 299 (1968)Google Scholar
  14. Howard-Flanders, P., Theriot, L.: Mutants of Escherichia coli K-12 defective in DNA repair and genetic recombination. Genetics 53, 1137–1150 (1966)Google Scholar
  15. Konrad, E.B., Lehman, I.: A conditional lethal mutant of Escherichia coli K-12 defective in the 5′-3′ exonuclease associated with DNA polymerase I. Proc. nat. Acad. Sci. (Wash.) 71, 2048–2051 (1974)Google Scholar
  16. Lloyd, R.G.: Ph.D. Thesis, Sussex University (1972)Google Scholar
  17. Lloyd, R.G., Barbour, S.D.: The genetic location of the sbcA gene of Escherichia coli. Molec. gen. Genet. 134, 157–171 (1974)Google Scholar
  18. Lloyd, R.G., Low, B., Godson, G.N., Birge, E.A.: Isolation and characterisation of an Escherichia coli K-12 mutant with a temperature-sensitive RecA pheotype. J. Bact. 120, 407–415 (1974)Google Scholar
  19. Low, K.B.: Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J. Bact. 113, 798–812 (1973)Google Scholar
  20. Manly, K.F., Signer, E.R., Radding, C.M.: Nonessential function of bacteriophage λ. Virology 37, 177–188 (1969)Google Scholar
  21. Monk, M., Kinross, J.: Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic-acid polymerase 1. J. Bact. 109, 971–978 (1972)Google Scholar
  22. Mount, D.W., Low, K.B., Edminston, S.J.: Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. J. Bact. 112, 886–893 (1972)Google Scholar
  23. Oliver, P., Stacey, K.A.: A mutant of E. coli K-12 with an impairment in the formation of stable P1-mediated transductants. Soc. gen. Microbiol. Proc. 1, 12 (1973)Google Scholar
  24. Stacey, K.A., Simson, E.: Improved method for the isolation of thymine-requiring mutants of Escherichia coli. J. Bact. 90, 554–555 (1965)Google Scholar
  25. Storm, P.K., Hoekstra, W.P.M., De Haan, P.G., Verhoef, C.: Genetic recombination in Escherichia coli. IV. Isolation and characterisation of recombination-deficient mutants of E. coli K-12. Mutation Res 13, 9–17 (1971)Google Scholar
  26. Takahashi, I., Barnard, R.A.: Effect of N-methyl-N′-Nitro-N-Nitrosoguanidine on the F factor of Escherichia coli. Mutation Res. 4, 111–117 (1967)Google Scholar
  27. Taylor, A., Trotter, C.D.: Linkage map of Escherichia coli strain K-12. Bact. Rev. 36, 504–524 (1972)Google Scholar
  28. Wilkins, B.M.: Chromosome transfer from F-lac + strains of Escherichia coli K-12 mutant at recA, recB, or recC. J. Bact 98, 599–604 (1969)Google Scholar
  29. Zissler, J., Signer, E.R. Schaefer, F.: In A.D. Hersley (ed.), the bacteriophage lambda, p. 455. (1971)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • K. A. Stacey
    • 1
  • Robert G. Lloyd
    • 1
  1. 1.School of Biological SciencesUniversity of SussexBrightonEngland
  2. 2.Biological LaboratoryThe UniversityCanterbury
  3. 3.Genetics DepartmentUniversity of NottinghamNottingham

Personalised recommendations