Theoretical and Applied Genetics

, Volume 78, Issue 6, pp 879–883 | Cite as

Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture

  • I. K. D. Tuvesson
  • S. Pedersen
  • S. B. Andersen


Inheritance of the ability to respond in wheat anther culture was studied from 6×2 reciprocal crosses between six varieties with high and two varieties with low capacity for green plant formation and their parents, replicated in two environments. Effects of genotypes dominated embryo formation and percentages of green plants, accounting for 78.4% and 85.4% of total variation, respectively, while smaller genetic effects were indicated for regeneration. Nuclear genes could explain almost all the genotype effects in this material. Embryo formation showed heterosis over high parent for 5 of the 12 hybrids, while percentages of green plants from the hybrids were intermediate to the parents. General Combining Ability (GCA) could explain 78.8% of the variation for embryo formation among the hybrids, whereas differences in percentage of green plants were dominated by Specific Combining Ability (SCA), accounting for 67.9% of hybrid variation. A positive correlation (r=0.81**) was observed between the genetic capacity for regeneration and green plant formation. Analysis of covariance indicated that effects causing GCA for green plant formation were mainly responsible for this correlation. A regression model with two parallel lines divided the six parent lines with high green plant formation into three groups with respect to their reactions with the two testers. The results are discussed with regard to possible involvement of two sets of nuclear genes affecting the percentage of green plants obtained in wheat anther culture: one set consisting of mainly additive effects affecting green plant percentage through an initial effect on regeneration ability, and another set of two or a few more major genes with dominance or epistatic effects uncorrelated with regeneration.

Key words

Wheat Anther culture Androgenetic responsiveness Albinism Genetic control 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Andersen SB, Due IK, Olesen A (1987) The response of anther culture in a genetically wide material of winter wheat (Triticum aestivum L.). Plant Breed 99:181–186Google Scholar
  2. Bullock WP, Baenziger PS, Schaeffer GW, Bottino PJ (1982) Anther culture of wheat (Triticum aestivum L.) F1's and their reciprocal crosses. Theor Appl Genet 62:155–159Google Scholar
  3. Charmet G, Bernard S (1984) Diallel analysis of androgenetic plant production in hexaploid Triticale (X. triticosecale, Wittmack). Theor Appl Genet 69:55–61Google Scholar
  4. Day A, Ellis THN (1984) Chloroplast DNA deletions associated with wheat plants regenerated from pollen: Possible basis for maternal inheritance of chloroplasts. Cell 39:359–368Google Scholar
  5. Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Genet 9:671–678Google Scholar
  6. Deaton WR, Metz SG, Armstrong TA, Mascia PN (1987) Genetic analysis of the anther-culture response of three spring wheat crosses. Theor Appl Genet 74:334–338Google Scholar
  7. Dunwell JM, Francis RJ, Powell W (1987) Anther culture of Hordeum vulgare L.: a genetic study of microspore callus production and differentiation. Theor Appl Genet 74:60–64Google Scholar
  8. Foroughi-Wehr B, Friedt W (1984) Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor Appl Genet 67:377–382Google Scholar
  9. Foroughi-Wehr B, Friedt W, Wenzel G (1982) On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor Appl Genet 62:233–239Google Scholar
  10. Henry Y, De Buyser J (1985) Effect of the 1B/1R translocation on anther culture ability in wheat (Triticum aestivum L.). Plant Cell Rep 4:307–310Google Scholar
  11. Knudsen S, Due IK, Andersen SB (1989) Components of response in barley anther culture. Plant Breed (in press)Google Scholar
  12. Lazar MD, Baenziger PS, Schaeffer GW (1984) Combining abilities and heritability of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther cultures. Theor Appl Genet 68:131–134Google Scholar
  13. Ouyang JW, Zhou SM, Jia SE (1983) The response of anther culture to culture temperature in Triticum aestivum. Theor Appl Genet 66:101–109Google Scholar
  14. Powell W (1988) Diallel analysis of barley anther culture response. Genome 30:152–157Google Scholar
  15. Wang Xingzhi, Hu Han (1984) The effect of potato II medium for triticale anther culture. Plant Sci Lett 36:237–239Google Scholar
  16. Wenzel G, Hoffmann F, Thomas E (1977) Increased induction and chromosome doubling of androgenetic haploid rye. Theor Appl Genet 51:81–86Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • I. K. D. Tuvesson
    • 1
  • S. Pedersen
    • 1
  • S. B. Andersen
    • 1
  1. 1.Department of Crop ScienceThe Royal Veterinary and Agricultural UniversityFrederiksberg CDenmark

Personalised recommendations