Histochemistry

, Volume 94, Issue 4, pp 381–386

Immunohistochemical localization of carbonic anhydrase isoenzymes in the human male reproductive tract

  • K. Kaunisto
  • S. Parkkila
  • T. Tammela
  • L. Rönnberg
  • H. Rajaniemi
Article

DOI: 10.1007/BF00266444

Cite this article as:
Kaunisto, K., Parkkila, S., Tammela, T. et al. Histochemistry (1990) 94: 381. doi:10.1007/BF00266444

Summary

The distribution of human carbonic anhydrase (HCA) isoenzymes I, II and VI in the human male reproductive tract was studied using specific antisera against affinity purified isoenzymes in conjunction with the peroxidase-antiperoxidase complex method. HCA VI-specific staining could not be demonstrated in any of the tissues studied, and HCA I was observed only in red blood cells. Immunostaining denoted HCA II in the epithelia of the seminal vescle, ampulla of the ductus deferens and distal ductus deferens. Some cells in the epithelium of the corpus and cauda epididymidis also stained for HCA II. The staining for HCA II in the epithelium of the reproductive tract declined from the strongly positive seminal vesicle to the proximal part of the ductus deferens, which stained negatively. There were also HCA II-positive particles derived from the apical protrusions of the epithelium in the lumina of the seminal vesicle, ampulla of the ductus deferens and ductus deferens. The physiological role of HCA II is linked to the secretion of bicarbonate into the seminal plasma and thereby to the regulation of sperm motility and pH in the seminal plasma.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • K. Kaunisto
    • 1
  • S. Parkkila
    • 1
  • T. Tammela
    • 2
  • L. Rönnberg
    • 3
  • H. Rajaniemi
    • 1
  1. 1.Department of AnatomyUniversity of OuluOuluFinland
  2. 2.Division of Urology, Department of SurgeryUniversity of OuluFinland
  3. 3.Department of Gynaecology and ObstetricsUniversity of OuluFinland

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