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Molecular and General Genetics MGG

, Volume 232, Issue 3, pp 423–426 | Cite as

Cloning of the mating-type gene MATA of the yeast Yarrowia lipolytica

  • Cornelia Kurischko
  • Philippe Fournier
  • Marion Chasles
  • Herbert Weber
  • Claude Gaillardin
Article
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Summary

The mating type gene MA TA of the dimorphic yeast Yarrowia lipolytica was cloned. The strategy used was based on the presumed function of this gene in the induction of sporulation. A diploid strain homozygous for the mating type B was transformed with an integrative gene bank from an A wild-type strain. A sporulating transformant was isolated, which contained a plasmid with an 11.6 kb insert. This sequence was rescued from the chromosomal DNA of the transformant and deletion mapping was performed to localize the MAT insert. The MAT gene conferred both sporulating and non-mating phenotypes on a B/B diploid. A LEU2 sequence targeted to this locus segregated like a mating type-linked gene. The A strain did not contain silent copies of the MAT gene.

Key words

Mating type Yarrowia lipolytica Protoplast fusion Sporulation Conjugation 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Cornelia Kurischko
    • 1
  • Philippe Fournier
    • 2
  • Marion Chasles
    • 2
  • Herbert Weber
    • 1
  • Claude Gaillardin
    • 2
  1. 1.Institut für Mikrobiologie und experimentelle Therapie (ZIMET)BeutenbergstrasseGermany
  2. 2.Institut National de la Recherche AgronomiqueGrignonFrance

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