Applied Microbiology and Biotechnology

, Volume 29, Issue 4, pp 323–328 | Cite as

Model for continuous production of solvents from whey permeate in a packed bed reactor using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar

  • N. Qureshi
  • A. H. J. Paterson
  • I. S. Maddox
Biotechnology

Summary

A mathematical model has been developed to describe the operation of a packed bed reactor for the continuous production of solvents from whey permeate. The model has been used to quantitate the amounts of different physiological/ morphological types of biomass present in the reactor. The majority of biomass is inert, i.e. it neither grows nor produces solvent. Only relatively small amounts of biomass actively grow (vegetative, non-solvent-producing cells), while even smaller amounts are responsible for solvent production (clostridial, solvent-producing cells).

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References

  1. Bailey JE, Ollis DF (1986) Biochemical Engineering Fundamentals. McGraw-Hill, New York, p 207Google Scholar
  2. Coulson JM, Richardson JF (1983) Chemical Engineering, Vol. 6. Pergamon Press Oxford P255Google Scholar
  3. Ennis BM (1987) Intensification of the acetone:butanol:ethanol fermentation using whey permeate and Clostridium acetobutylicum. Ph. D. thesis, Massey University, New ZealandGoogle Scholar
  4. Ennis BM, Maddox IS (1985) Use of Clostridium acetobutylicum P262 for production of solvents from whey permeate. Biotechnol Lett 7:601–606Google Scholar
  5. Ennis BM, Maddox IS (1987) The effect of pH and lactose concentration on solvent production from whey permeate using Clostridium acetobutylicum. Biotechnol Bioeng 29:329–334Google Scholar
  6. Ennis BM, Maddox IS Schoutens GH (1986) Immobilized Clostridium acetobutylicum for continuous butanol production from whey permeate. New Zealand J Dairy Science Technol 21:99–109Google Scholar
  7. Forberg C, Enfors S-O, Häggström L (1983) Control of immobilized non-growing cells for continuous production of metabolites. Eur J Appl Microbiol Biotechnol 17:143–147Google Scholar
  8. Häggström L, Forberg C (1986) Significance of an extracellular polymer for the energy metabolism in Clostridium acetobutylicum: a hypothesis. Appl Microbiol Biotechnol 23:234–239Google Scholar
  9. Häggström L, Molin N (1980) Calcium alginate immobilized cells of Clostridium acetobutylicum for solvent production. Biotechnol Lett 2:241–246Google Scholar
  10. Jones DT, van der Westhuizen A, Long S, Allcock ER, Reid SJ, Woods DR (1982) Solvent production and morphological changes in Clostridium acetobutylicum. Appl Environ Microbiol 43:1434–1439Google Scholar
  11. Krouwel PG, van der Laan WFM, Kossen NWF (1980) Continuous production of n-butanol and isopropanol by immobilized, growing Clostridium acetobutylicum cells. Biotechnol Lett 2:253–258Google Scholar
  12. Largier ST, Long S, Santangelo JD, Jones DT, Woods DR (1985) Immobilized Clostridium acetobutylicum P262 mutants for solvent production. Appl Environ Microbiol 50:477–481Google Scholar
  13. Pirt SJ (1975) Principles of microbe and cell cultivation. Black-well Scientific Publications, Oxford, UKGoogle Scholar
  14. Qureshi N, Maddox IS (1987) Continuous solvent production from whey permeate using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Enz Microbial Technol 9:668–671Google Scholar
  15. Schoutens GH, van Beelen PN, Luyben K ch AM (1986) A simple model for continuous production of butanol by immobilized Clostridia I. Chem Eng J (Lausanne) 32:B43–50Google Scholar
  16. Treybal RE (1981) Mass transfer operations. Third edition, McGraw Hill International Book Company, AucklandGoogle Scholar
  17. Yerushalmi L, Volesky B, Leung WK, Neufeld RJ (1983) Variations of solvent yield in acetone butanol fermentation. Eur J Appl Microbiol Biotechnol 18:279–286Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • N. Qureshi
    • 1
  • A. H. J. Paterson
    • 1
  • I. S. Maddox
    • 1
  1. 1.Biotechnology DepartmentMassey UniversityPalmerston NorthNew Zealand

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