Theoretical and Applied Genetics

, Volume 59, Issue 3, pp 177–184 | Cite as

Basis and extent of genetic variability among doubled haploid plants obtained by pollen culture in Nicotiana sylvestris

  • R. De Paepe
  • D. Bleton
  • F. Gnangbe


All diploid plants (doubled-haploid plants: D.H.) regenerated by androgenesis from binucleated pollen grains in Nicotiana sylvestris differ genetically from the original line as far as morphological features and growth rates are concerned. This androgenic variation (A.V.) is under nuclear control and is transmitted continuously by some D.H. for at least four generations of selfing; other D.H. progenies segregate. Further androgeneses carried out on one single D.H. reveal a new variability and increase the drift from the original line. All results cannot be explained by the presence of residual heterozygosity in the original line, and we suggest that most of the A.V. could originate from changes that occur in the DNA of the vegetative pollen grain cell. D.H. resulting from endomitosis of the vegetative cell would be ‘homozygous’ and stable, whereas D.H. resulting from nuclear fusion between a vegetative and a generative cell would be ‘heterozygous’ and would segregate in seeds through succeeding generations.

Key words

Androgenesis Doubled-haploid (D.H.) Mendelian segregation Quantitative variation 


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  1. Arcia, M.A.; Wernsman, E.A.; Burk, L.G. (1978): Performance of anther-derived dihaploids and their conventionally inbred parents as lines, in F1 hybrids and in F2 generations. Crop Sci. 18, 413–418Google Scholar
  2. d'Amato, F. (1977): Cytogenetics of differentiation in tissue ancell cultures. In: Plant cell, tissue and organ culture (ed.: Reinert, J.; Bajaj, Y.P.S., pp. 342–357 Berlin, Heidelberg, New York, SpringerGoogle Scholar
  3. Bourgin, J.P.; Nitsch, J.P. (1967): Obtention de Nicotiana haploides à partir d'étamines cultivées in vitro. Ann. Physiol. Vég. 9 (4), 377–382Google Scholar
  4. Burk, L.G.; Matzinger, D.F. (1976): Variation among anther derived doubled-haploids from an inbred line of tobacco. J. Hered. 67, 381–384Google Scholar
  5. Collins, G.B.; Legg, P.D. (1974): Breeding allopolyploids. In: Haploids in higher plants (ed.: Kasha, K.S.), pp. 231–247. Guelph. Ontario: Univ. Guelph PressGoogle Scholar
  6. De Paepe, R. (1977): Modifications héréditaires présentées par des haploides doublés obtenus par androgenèse chez N. sylvestris. Thèse de 3è cycle, Univ. Paris XI OrsayGoogle Scholar
  7. De Paepe, R.; Pernes, J. (1978): Exemples de variations à hérédité mendélienne induites au cours du développement des plantes. Physiol. Vég. 16 (2), 195–204Google Scholar
  8. De Paepe, R.; Nitsch, C.; Godard, M.; Pernes, J. (1976): Potential from haploid and possible use in agriculture. In: Plant tissue culture and its biotechnological application, pp. 341–352. Berlin, Heidelberg, New York: SpringerGoogle Scholar
  9. Devreux, M.; Saccardo, F. (1972): Ibridazine di linee isogenische di tobacco obtenute da cultura in vitro di antere. Genet. Agraria 26, 143–146Google Scholar
  10. Engvild, K.C. (1974): Plantlet ploidy and flower bud size in tobacco anther culture. Hereditas 76, 320–322Google Scholar
  11. Jensen, C.S. (1974): Chromosome doubling techniques in haploids. In: Haploids in higher plants (ed.: Kasha, K.S.) pp. 153–190. Guelph, Ontario: Univ. Guelph PressGoogle Scholar
  12. Kameya, T.; Hinata, K. (1970): Induction of haploid plants from pollen grains of Brassica. Jpn. J. Breed. 20, 82–87Google Scholar
  13. Knop, W. (1865): Quantitative Untersuchung über den Ernährungs-Process der Pflanzen. Landw. Versuchsstat. 7, 93107Google Scholar
  14. Lauth, M.R.; Spear, B.B.; Heumann, J.; Prescott, D.M. (1976): DNA of ciliated protozea: DNA sequence diminution during macronuclear development in Oxytricha. Cell 7, 67–74Google Scholar
  15. Lin, M.L.; Stabas (1961): Peppermint and spearmint tissue culture. In: Callus formation in submerged culture. Lloydia, 24, 139–145.Google Scholar
  16. McComb, J.A.; McComb, A.J. (1977): The cytology of plantlets derived from cultured anthers of Nicotiana sylvestris. New Phytol. 79, 679–688Google Scholar
  17. Niizeki, H.; Oono, K. (1968): Induction of haploid rice plant from anther culture. Proc. Jpn. Acad. 44, 554–557.Google Scholar
  18. Nitsch, C. (1974): Pollen culture. A New technique for mass production of haploids and homozygous plants. In: proc. int. symp. on haploid in higher-plants (ed.: Kasha, K.S.), pp. 123–135. Guelph, Ontario: Univ. Guelph PressGoogle Scholar
  19. Nitsch, C. (1977): Culture of isolated microspores. In: plant cell, tissue and organ culture (ed.: Reinert, J.; Bajaj, Y.P.S.), pp. 268–278. Berlin, Heidelberg, New York: SpringerGoogle Scholar
  20. Nitsch, C.; Norreel, B. (1973): Effet d'un choc thermique sur le pouvoir embryogène du pollen de Datura innoxia cultivé dans l'anthère ou isolé de l'anthère. C.R. Acad. Sci. Paris, 276 (D), 303–306Google Scholar
  21. Nitsch, J.P. (1968): Les phytotrons et la bioclimatologie experimentale. Encycl. Pléiade, Vol. physiologie, pp. 1602–1617. Paris: GallimardGoogle Scholar
  22. Oinuma, T.; Yoschida, T. (1975): Genetic variation among doubled haploid lines of burley tobacco varieties. Jpn. J. Breed. 24 (5), 211–216Google Scholar
  23. Raschid, A.; Street, H.E. (1974): Segmentation in microspores of Nicotiana sylvestris and Nicotiana tabacum which lead to embryoid formation in anther cultures. Protoplasma 80, 323–334Google Scholar
  24. Ross, J.G. (1965): Somatic chromosome reduction and spectrum mutational effects after colchicine treatment of sorghum. In: The use of induced mutations in plant breeding. pp. 193–203. FAO/IAEA meeting Rome 1964. London: Pergamon PressGoogle Scholar
  25. Scheffé, H. (1959): The analysis of variance. London: WileyGoogle Scholar
  26. Sunderland, N.; Collins, G.B.; Dunwell, J.M. (1974): The role of nuclear fusion in pollen embryogenesis of Datura innoxia Müll. Planta 117, 227–241Google Scholar
  27. Truong, I. (1977): Variabilité des plantes issues d'androgenèse in vitro et tentative d'application directe en sélection de cette variabilité et de la mutagenèse par voie haploide chez le Riz (Oryza sativa). Thèse 3è Cyclce, Univ. Paris XI OrsayGoogle Scholar
  28. Wenzel, G.; Hoffmann, F.; Thomas, E. (1976): Heterozygous microspore derived plants in rye. Theor. Appl. Genet. 48, 205–208Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • R. De Paepe
    • 1
  • D. Bleton
    • 1
  • F. Gnangbe
    • 1
  1. 1.Laboratoire Génétique et Physiologie du Développement des PlantesCNRSGif-sur-YvetteFrance

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