Advertisement

Theoretical and Applied Genetics

, Volume 59, Issue 3, pp 161–167 | Cite as

Mitochondrial DNA variation in maize plants regenerated during tissue culture selection

  • B. G. Gengenbach
  • J. A. Connelly
  • D. R. Pring
  • M. F. Conde
Article

Summary

Plants resistant to Helminthosporium maydis race T were obtained following selection for H. maydis pathotoxin resistance in tissue cultures of susceptible, Texas male-sterile (T) cytoplasm maize. The selected lines transmitted H. maydis resistance to their sexual progeny as an extranuclear trait. Of 167 resistant, regenerated plants, 97 were male fertile and 70 were classified male sterile for reasons that included abnormal plant, tassel, anther or pollen development. No progeny were obtained from these male-sterile, resistant plants. Male fertility and resistance to the Phyllosticta maydis pathotoxin that specifically affects T cytoplasm maize were co-transmitted with H. maydis resistance to progeny of male-fertile, resistant plants. These three traits previously were associated only with the normal (N) male-fertile cytoplasm condition in maize. Three generations of progeny testing provided no indication that the cytoplasmic association of male sterility and toxin susceptibility had been broken by this selection and regeneration procedure. Restriction endonuclease analysis of mitochondrial DNA (mtDNA) revealed that three selected, resistant lines had distinct mtDNA organization that distinguished them from each other, from T and from N cytoplasm maize. Restriction patterns of the selected resistant lines were similar to those from T cytoplasm mtDNA; these patterns had not been observed in any previous analyses of various sources of T cytoplasm. The mtDNA analyses indicated that the male-fertile, toxin-resistant lines did not originate from selection of N mitochondrial genomes coexisting previously with T genomes in the T cytoplasm line used for selection.

Key words

Extranuclear inheritance Male sterile cytoplasm Helminthosporium maydis resistance Maize tissue culture Mitochondrial DNA analysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature

  1. Belliard, G.; Vedel, F., Pelletier, G. (1979): Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281, 401–403Google Scholar
  2. Berville, A. (1978): Characterization of mitochondria from mutants resistant to Helminthosporium maydis race T. In: Plant mitochondria (eds. Ducet, G.; Lance, C.), pp. 427–434. Amsterdam-Shannon-New York: Elsevier/North-Holland Biomedical PressGoogle Scholar
  3. Brettell, R.I.S.; Goddard, B.V.D.; Ingram, D.S. (1979): Selection of Tms-cytoplasm maize tissue cultures resistant to Drechslera maydis T-toxin. Maydica 24, 203–312Google Scholar
  4. Cassini, R.; Cornu, A.; Berville, A.; Vuillaume, E.; Panouille, A. (1977): Heredite et caracteristiques des sources de resistance a Helminthosporium maydis race T, obtenues par mutagenese chez des mais a cytoplasme male sterile Texas. Ann. Amelior. Plant. 27, 753–766Google Scholar
  5. Comstock, J.C.; Martinson, C.A.; Gengenbach, B.G. (1973): Host specificity of a toxin from Phyllosticta maydis for Texas cytoplasmically male-sterile maize. Phytopathology 63, 1357–1361Google Scholar
  6. Cornu, A.; Cassini, R.; Berville, A.; Vuillaume, E. (1977): Recherche par mutagenese d'une resistance a Helminthosporium maydis, race T, chez les mais a cytoplasme male-sterile Texas. In: Induced Mutations Against Plant Diseases, pp. 479–488. Vienna: Intern. Atomic Energy AgencyGoogle Scholar
  7. Forde, B.G.; Leaver, C.J. (1980): Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc. Nat. Acad. Sci. (Wash.) 77, 418–422Google Scholar
  8. Forde, B.G.; Oliver, R.J.C.; Leaver, C.J. (1978): Variation in mitochondrial translation products associated with male-sterile cytoplasms in make. Proc. Nat. Acad. Sci. (Wash.) 75, 3841–3845Google Scholar
  9. Gengenbach, B.G.; Green, C.E. (1975): Selection of T-cytoplasm maize callus cultures resistant to Helminthosporium maydis race T pathotoxin. Crop Sci. 15, 645–649Google Scholar
  10. Gengenbach, B.G.; Green, C.E.; Donovan, C.D. (1977): Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc. Nat. Acad. Sci. (Wash.) 74, 5113–5117Google Scholar
  11. Green, C.E. (1977): Prospects for crop improvement in the field of cell culture. Hort. Sci. 12, 131–134Google Scholar
  12. Green, C.E.; Phillips, R.L. (1975): Plant regeneration from tissue cultures of maize. Crop Sci. 15, 417–421Google Scholar
  13. Hooker, A.L.; Smith, D.R.; Lim, S.M.; Beckett, J.B. (1970): Reaction of corn seedlings with male-sterile cytoplasm to Helminthosporium maydis. Plant Dis. Reptr. 54, 708–712Google Scholar
  14. Kemble, R.J.; Bedbrook, J.R. (1980): Low molecular weight circular and linear DNA molecules in mitochondria from normal and male-sterile cytoplasms of Zea mays. Nature 284, 565–566Google Scholar
  15. Laughnan, J.R.; Gabay, S.J. (1973): Mutations leading to nuclear restoration of fertility in S male-sterile cytoplasm in maize. Theor. Appl. Genet. 43, 109–116Google Scholar
  16. Levings, C.S., III; Pring, D.R. (1976): Restriction endonuclease analysis of mitochondrial DNA from normal and Texas cytoplasmic male-sterile maize. Science 193, 158–160Google Scholar
  17. Levings, C.S., III; Pring, D.R.; Conde, M.F.; Laughnan, J.R.; Gabay-Laughnan, S.J. (1979a): The basis for cytoplasmic instability in S cytoplasm. Maize Genet. Newslett. 53, 83–84Google Scholar
  18. Levings, C.S., III; Shah, D.M.; Hu, W.W.L.; Pring, D.R.; Timothy, D.H. (1979b): Molecular heterogeneity among mitochondrial DNAs from different maize cytoplasms. In: Extrachromosomal DNA (ICN-UCLA Symposia), pp. 63–74. New York: Acad. PressGoogle Scholar
  19. Lim, S.M.; Hooker, A.L. (1972): Disease determinant of Helminthosporium maydis race T. Phytopathology 62, 968–971Google Scholar
  20. Pring, D.R.; Levings, C.S., III (1978): Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89, 121–136Google Scholar
  21. Pring, D.R.; Levings, C.S., III; Hu, W.W.L.; Timothy, D.H. (1977): Unique DNA associated with mitochondria in the ‘S’-type cytoplasm of male-sterile maize. Proc. Nat. Acad. Sci. (Wash.) 74, 2904–2908Google Scholar
  22. Quetier, F.; Vedel, F. (1977): Heterogeneous populations of mitochondrial DNA molecules in higher plants. Nature 268, 365–368Google Scholar
  23. Scheifele, G.L.; Whitehead, W.; Rowe, C. (1970): Increased susceptibility to southern leaf spot (Helminthosporium maydis) in inbred lines and hybrids of maize with Texas male-sterile cytoplasm. Plant Dis. Reptr. 54, 501–503Google Scholar
  24. Singh, A.; Laughnan, J.R. (1972): Instability of S male-sterile cytoplasm in maize. Genetics 71, 607–620Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • B. G. Gengenbach
    • 1
  • J. A. Connelly
    • 1
    • 4
  • D. R. Pring
    • 2
  • M. F. Conde
    • 3
  1. 1.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  2. 2.USDA/SEA/AR, Department of Plant PathologyUniversity of FloridaGainesvilleUSA
  3. 3.Department of Plant PathologyUniversity of FloridaGainesvilleUSA
  4. 4.Department of BiochemistryUniversity of CaliforniaDavisUSA

Personalised recommendations