Molecular and General Genetics MGG

, Volume 131, Issue 4, pp 281–289 | Cite as

Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether

  • Ian W. Dawes
  • Ian D. Hardie


Ascospores of Saccharomyces cerevisiae are considerably less sensitive to ether than vegetative yeast. Sporulated cultures grown on either liquid or solid media can be treated with ether to selectively kill vegetative organisms. This differential sensitivity of vegetative cells can be exploited to advantage in a number of genetic manipulations. These include: random spore analysis; selecting mutants affected in the developmental processes of sporulation and spore germination; detection of sporulating revertants or recombinants from non-sporulating diploids; determination of the incidence of spores present in cultures at frequencies as low as 1 per 106 viable organisms; and, complementation testing of developmental mutations.


Diethyl Vegetative Cell Diethyl Ether Saccharomyces Cerevisiae Saccharomyces 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Balassa, G.: The genetic control of sporeformation in bacilli. Curr. Top. Microbiol. Immunol. 56, 99 (1971)Google Scholar
  2. Emeis, V., Gutz, H.: Eine einfache Technik zur Massenisolation von Hefersporen. Z. Naturforsch. 13b, 647 (1958)Google Scholar
  3. Esposito, M. S., Esposito, R. E.: The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants. Genetics 61, 79 (1969)Google Scholar
  4. Fast, D.: Sporulation synchrony of Saccharomyces cerevisiae grown in various carbon sources. J. Bact. 116, 925 (1973)Google Scholar
  5. Fowell, R. R.: Sporulation and hybridization of yeasts. In: The yeasts (A. H. Rose and J. S. Harrison, eds.), vol. 1, p. 303. London-New York: Academic Press 1969Google Scholar
  6. Johnston, J. R., Mortimer, R. K.: Use of snail digestive juice in isolation of yeast spore tetrads. J. Bact. 78, 292 (1959)Google Scholar
  7. Leupold, U.: Physiologisch-genetische Studien an adenin-abhängigen Mutanten von Schizosaccharomyces pombe. Ein Beitrag zum Problem der Pseudoallelie. Schweiz. Z. Path. Bakt. 20, 535 (1957)Google Scholar
  8. Magni, G. E., Borstel, R. C. von: Different rates of spontaneous mutation during mitosis and meiosis in yeast. Genetics 47, 1097 (1962)Google Scholar
  9. Murrell, W. G.: Biochemistry of the bacterial endospore. Adv. microbial Physiol. 1, 133 (1967)Google Scholar
  10. Resnick, M. A., Tippetts, R. D., Mortimer, R. K.: Separation of spores from diploid cells of yeast by stable flow free-boundary electrophoresis. Science 158, 803 (1967)Google Scholar
  11. Rousseau, P., Halvorson, H. O.: Preparation and storage of single spores of Saccharomyces cerevisiae. J. Bact. 100, 1426 (1969)Google Scholar
  12. Sherman, F., Roman, H.: Evidence for two types of allelic recombination in yeast. Genetics 48, 255 (1963)Google Scholar
  13. Vary, J., Kornberg, A.: Biochemical studies of bacterial sporulation and germination. XXI. Temperature-sensitive mutants for initiation of germination. J. Bact. 101, 327 (1970)Google Scholar
  14. Waites, W. M., Kay, G., Dawes, I. W., Wood, D. A., Warren, S. C., Mandelstam, J.: Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants. Biochem. J. 118, 667 (1970)Google Scholar
  15. Zakharov, I. A., Inge-Vechtomov, S. G.: Ascospore isolation of yeast for genetic analysis without a micromanipulator. Issledovania po Genetike (English translation) 2, 134 (1964)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Ian W. Dawes
    • 1
  • Ian D. Hardie
    • 1
  1. 1.Department of MicrobiologyUniversity of EdinburghEdinburghScotland

Personalised recommendations