Molecular and General Genetics MGG

, Volume 131, Issue 4, pp 281–289 | Cite as

Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether

  • Ian W. Dawes
  • Ian D. Hardie
Article

Summary

Ascospores of Saccharomyces cerevisiae are considerably less sensitive to ether than vegetative yeast. Sporulated cultures grown on either liquid or solid media can be treated with ether to selectively kill vegetative organisms. This differential sensitivity of vegetative cells can be exploited to advantage in a number of genetic manipulations. These include: random spore analysis; selecting mutants affected in the developmental processes of sporulation and spore germination; detection of sporulating revertants or recombinants from non-sporulating diploids; determination of the incidence of spores present in cultures at frequencies as low as 1 per 106 viable organisms; and, complementation testing of developmental mutations.

Keywords

Diethyl Vegetative Cell Diethyl Ether Saccharomyces Cerevisiae Saccharomyces 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Ian W. Dawes
    • 1
  • Ian D. Hardie
    • 1
  1. 1.Department of MicrobiologyUniversity of EdinburghEdinburghScotland

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