Molecular and General Genetics MGG

, Volume 223, Issue 3, pp 513–516 | Cite as

A previously unrecognized glutamine synthetase expressed in Klebsiella pneumoniae from the glnT locus of Rhizobium leguminosarum

  • G. Espin
  • S. Moreno
  • M. Wild
  • R. Meza
  • M. Iaccarino
Short Communications

Summary

Using glnT DNA of Rhizobium meliloti as a hybridization probe we identified a R. leguminosarum biovar phaseoli (R. l. phaseoli) locus (glnT) expressing a glutamine synthetase activity in Klebsiella pneumoniae. A 2.2 kb DNA fragment from R. l. phaseoli was cloned to give plasmid pMW5a, which shows interspecific complementation of a K. pneumoniaeglnA mutant. The cloned sequence did not show cross-hybridization to glnA or glnII, the genes coding for two glutamine synthetase isozymes of Rhizobium spp. While in previous reports on glnT of R. meliloti and Agrobacterium tumefaciens no glutamine synthetase activity was detected, we do find activity with the glnT locus of R. l. phaseoli. The glutamine synthetase (GSIII) activity expressed in a K. pneumoniae glnA strain from pMW5a shows a ratio of biosynthetic to transferase activity 103-fold higher than that observed for GSI or GSII. GSIII is similar in molecular weight and heat stability to GSI.

Key words

Rhizobium leguminosarum bv. phaseoli Glutamine synthetases Nitrogen metabolism glnT 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • G. Espin
    • 1
  • S. Moreno
    • 1
  • M. Wild
    • 1
  • R. Meza
    • 1
  • M. Iaccarino
    • 2
  1. 1.Unidad de Biologia Molecular y Biotecnologia VegetalUniversidad Nacional Autonoma de MexicoCuernavacaMexico
  2. 2.CNRIstituto Internazionale di Genetica e BiofisicaNapoliItaly

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