Archives of Microbiology

, Volume 162, Issue 1–2, pp 85–90 | Cite as

Holophaga foetida gen. nov., sp. nov., a new, homoacetogenic bacterium degrading methoxylated aromatic compounds

  • Werner Liesack
  • Friedhelm Bak
  • Jan-Ulrich Kreft
  • E. Stackebrandt
Original Paper


A polyphasic approach was used in which genotypic and phenotypic properties of a gram-negative, obligately anaerobic, rod-shaped bacterium isolated from a black anoxic freshwater mud sample were determined. Based on these results, the name Holophaga foetida gen. nov., sp. nov. is proposed. This microorganism produced dimethylsulfide and methanethiol during growth on trimethoxybenzoate or syringate. The only other compounds utilized were pyruvate and trihydroxybenzenes such as gallate, phloroglucinol, or pyrogallol. The aromatic compounds were degraded to acetate. Although comparison of the signature nucleotide pattern of the five established subclasses of Proteobacteria with the 16S rDNA sequence of Holophaga foetida revealed a relationship to members of the δ-subclass, the phylogenetic position within the radiation of this class is so deep and dependent upon the number and selection of reference sequences that its affiliation to the Proteobacteria must be considered tentative. The type strain is H. foetida strain TMBS4 (DSM 6591).

Key words

Holophaga foetida Sulfide-methylation Homoacetogenic bacterium Pelobacter Acetobacterium 16S rRNA analysis Phylogeny 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Werner Liesack
    • 1
  • Friedhelm Bak
    • 1
  • Jan-Ulrich Kreft
    • 2
  • E. Stackebrandt
    • 3
  1. 1.Max-Planck-Institut für Terrestrische MikrobiologieMarburgGermany
  2. 2.Lehrstuhl für Mikrobielle ÖkologieUniversität KonstanzKonstanzGermany
  3. 3.Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM)BraunschweigGermany

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