Applied Microbiology and Biotechnology

, Volume 30, Issue 5, pp 440–443

Production of 2,3-butanediol by Klebsiella oxytoca

  • N. Qureshi
  • M. Cheryan

DOI: 10.1007/BF00263847

Cite this article as:
Qureshi, N. & Cheryan, M. Appl Microbiol Biotechnol (1989) 30: 440. doi:10.1007/BF00263847


High glucose concentrations result in high levels of 2,3-butanediol, improved yield and productivity, and a decrease in cell growth in batch cultures of Klebsiella oxytoca. A maximum of 84.2 g butanediol/l and a yield of 0.5 was obtained with an initial glucose concentration of 262.6g/l. Adding the substrate in two steps in a modified fed-batch operation resulted in 85.5 g butanediol/l, 6.4 g acetoin/l and 3.4 g ethanol/l with a net yield of 0.5. Increasing the cell density to 60g/l resulted in productivities as high as 3.22 g/l.h.

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • N. Qureshi
    • 1
  • M. Cheryan
    • 1
  1. 1.Biochemical Engineering LaboratoryUniversity of IllinoisUrbanaUSA

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