Journal of Comparative Physiology B

, Volume 163, Issue 2, pp 118–122 | Cite as

Glucose metabolism by brown trout peripheral blood lymphocytes

  • J. L. Albi
  • J. Planas
  • J. Sánchez
Article
Accepted:

Abstract

Glucose metabolism in peripheral blood lymphocytes from the brown trout Salmo trutta has been studied. Glucose is taken up by means of a sodium-independent saturable process (Km=10.8 mmol·l-1), as well as by simple diffusion. Once within the cell, most of glucose is directed to lactate production through either the Embden-Meyerhof pathway or the hexose-monophosphate shunt. Rates of lactate formation are higher than rates of CO2 formation. Glutamine does not exert an effect on either glucose uptake or glucose metabolism. The present study provides information regarding the nature of energy sources for different cell types in salmonids.

Key words

Phloretin-sensitive uptake Glucose metabolism Aerobic and anaerobic metabolism Peripheral blood lymphocytes Trout, Salmo trutta 

Abbreviations

3-OMG

3-O-methyl glucose

EM

Embden-Meyerhoff pathway

G6D

glucose-6-phosphate dehydrogenase

HK

hexokinase

HMS

hexose monophosphate shunt

ICDH

isocitrate dehydrogenase

Km

apparent Michaelis constant

LDH

lactate dehydrogenase

MCB

modified Cortland buffer

PBL

peripheral blood lymphocytes

PFK

fructose-6-phosphate kinase

PK

pyruvate kinase

RBC

red blood cells

Vmax

maximal rate of uptake

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References

  1. Ardawi MSM (1988) Glutamine and glucose metabolism in human peripheral lymphocytes. Metabolism 37:99–103Google Scholar
  2. Ardawi MSM, Newsholme EA (1982) Maximum activities of some enzymes of glycolysis, the tricarboxylic acid cycle and ketonebody and glutamine utilization pathways in lymphocytes of the rat. Biochem J 208:743–748Google Scholar
  3. Ardawi MSM, Newsholme EA (1983) Glutamine metabolism in lymphocytes of the rat. Biochem J 212:835–842Google Scholar
  4. Ardawi MSM, Newsholme EA (1984) Metabolism of ketone bodies, oleate and glucose in lymphocytes of the rat. Biochem J 221:255–260Google Scholar
  5. Argilés JM, López-Soriano FJ (1990) Why do cancer cells have such a high glycolytic rate? Medical Hypothesis 32:151–155Google Scholar
  6. Bartlett GR (1968) Phosphorus compounds in the human eruthrocyte. Biochim Biophys Acta 156:221–230Google Scholar
  7. Beutler E (1984) Red cell metabolism A manual of biochemical methods. Grunne and Stratton. Orlando, USAGoogle Scholar
  8. Brand K, Williams JF, Weidemann MJ (1984) Glucose and glutamine metabolism in rat thymocytes. Biochem J 221:471–475Google Scholar
  9. Culvenor JG, Weidemann MJ (1976) Phytohaemagglutinin stimulation of rat thymus lymphocyte glycolysis. Biochim Biophys Acta 437:354–363Google Scholar
  10. Ferguson RA, Storey KB (1991) Glycolytic and associated enzymes of rainbow trout (Onchorhynchus mykiss) red cells: in vitro and in vivo studies. J Exp Biol 155:469–485Google Scholar
  11. Hedeskov CJ (1968) Early effects of phytohaemagglutinin on glucose metabolism of normal human lymphocytes. Biochem J 110:373–380Google Scholar
  12. Houston AH, McCullough CAM, Keen J, Maddalena C, Edwards J (1985) Rainbow trout red cells in vitro. Comp Biochem Physiol 81A:555–565Google Scholar
  13. Hume DA, Radik JL, Feber E, Weidemann MJ (1978a) Aerobic glycolysis and lymphocyte transformation. Biochem J 174:703–709Google Scholar
  14. Hume DA, Hansen K, Weidemann MJ, Ferber E (1978b) Cytochalasin B inhibits lymphocyte transformation through its effects on glucose transport. Nature (London) 227:359Google Scholar
  15. Ingermann RL, Hall RE, Bissonnette JM, Terwilliger RC (1984) Monosaccharide transport into erythrocytes of the Pacific hagfish, Eptatretus stouti. Mol Physiol 6:311–320Google Scholar
  16. Knox D, Walton MJ, Cowey CB (1980) Distribution of enzymes of glycolysis and gluconeogenesis in fish tissues. Mar Biol 56:7–10Google Scholar
  17. Naftalin RJ, Rist RJ (1989) Evidence that activation of 2-deoxy-d-glucose transport in rat thymocyte suspensions results from enhanced coupling between transport and hexokinase activity. Biochem J 260:143–152Google Scholar
  18. Naftalin RJ, Rist RJ (1990) Effects of phorbol, dexamethasone and starvation on 3-O-methyl-d-glucose transport by rat thymocytes. Biochem J 265:251–259Google Scholar
  19. Navarro I, Canals P, Sánchez J, Gutiérrez J, Planas J (1991) Some plasma hormones and metabolites in the pyrenean brown trout (Salmo trutta fario). Comp Biochem Physiol 100A:919–923Google Scholar
  20. Newsholme P, Curi R, Gordon S, Newsholme EA (1986) Metabolism of glucose, glutamine, long-chain fatty acids and ketone bodies by murine macrophages. Biochem J 239:121–125Google Scholar
  21. O'Rourke AM, Rider CC (1989) Glucose, glutamine and ketone body utilization by resting and concanavalin A-activated rat splenic lymphocytes. Biochim Biophys Acta 1010:342–345Google Scholar
  22. Pesquero J, Albi JL, Gallardo MA, Planas J, Sánchez J (1992) Glucose metabolism by trout (Salmo trutta) red blood cells. J Comp Physiol B 162:448–454Google Scholar
  23. Peters JH, Haussen P (1971) Effect of phytohemagglutinin on lymphocyte membrane transport. 2. Stimulation of “facilitated diffusion” of 3-O-methyl-glucose. Eur J Biochem 19:509–513Google Scholar
  24. Planas J, Albi JL, Pesquero J, Sánchez J (1989) Glucose metabolism in brown trout erythrocytes (abstract). Arch Int Physiol 97:C41Google Scholar
  25. Reeves JP (1975) Stimulation of 3-O-methyl glucose transport by anaerobiosis in rat thymocytes. J Biol Chem 24:9413–9420Google Scholar
  26. Segal J (1987) The effect of trypsin on sugar uptake in rat thymocytes. Modulation of cellular cyclic AMP concentration and the sugar transport system. Biochem J 246:561–566Google Scholar
  27. Storey KB, Fields JHA (1988) NAD+-linked isocitrate dehydrogenase in fish tissues. Fish Physiol Biochem 5:1–8Google Scholar
  28. Tiihonen K, Nikinmaa M (1991a) Substrate utilization by carp (Cyprinus carpio) erythrocytes. J Exp Biol 161:509–514Google Scholar
  29. Tiihonen K, Nikinmaa M (1991b) d-Glucose permeability in river lamprey (Lampetra fluvialis) and carp (Cyprinus carpio) erythrocytes. Comp Biochem Physiol 100A:581–584Google Scholar
  30. Tse C-M, Young JD (1990) Glucose transport in fish erythrocytes: variable cytochalasin-B-sensitive hexose transport activity in the common eel (Anguilla japonica) and transport deficiency in the paddyfield eel (Monopterus albus) and rainbow trout (Salmo gairdnerii). J Exp Biol 148:367–383Google Scholar
  31. Walsh PJ, Wood CM, Thomas S, Perry SF (1990) Characterization of red blood cell metabolism in rainbow trout. J Exp Biol 154:475–489Google Scholar
  32. Walton MJ, Cowey BB (1982) Aspects of intermediary metabolism in salmonid fish. Comp Biochem Physiol 73B:59–79Google Scholar
  33. Wang T, Marquard C, Foker J (1976) Aerobic glycolysis during lymphocyte proliferation. Nature (London) 261:702–707Google Scholar
  34. Warburg O (1956) On the origin of cancer cells. Science 123:309–314Google Scholar
  35. Wheeler TJ, Hinkle PC (1985) The glucose transporter of mammalian cells. Annu Rev Physiol 47:503–518Google Scholar
  36. Yasmeen D, Laird AJ, Hume DA, Weidemann MJ (1977) Activation of 3-O-methyl-glucose transport in rat thymus lymphocytes by concanavalin A: temperature and calcium ion dependence and sensitivity to puromycin but not to cycloheximide. Biochim Biophys Acta 500:89–102Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • J. L. Albi
    • 1
  • J. Planas
    • 1
  • J. Sánchez
    • 1
  1. 1.Dpt. Bioquímica i Fisiologia, Unitat de FisiologiaUniversitat de BarcelonaBarcelonaSpain

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