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Theoretical and Applied Genetics

, Volume 67, Issue 5, pp 463–467 | Cite as

Flow-cytometric characterization and sorting of plant chromosomes

  • A. M. M. de Laat
  • J. Blaas
Article

Summary

Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.

Key words

Chromosome isolation Flow cytometry Haplopappus gracilis (Nutt.) Gray Synchronization 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • A. M. M. de Laat
    • 1
  • J. Blaas
    • 1
  1. 1.Research Institute ITALAA WageningenThe Netherlands

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