Theoretical and Applied Genetics

, Volume 54, Issue 4, pp 177–180 | Cite as

Gene-centromere mapping by 4x-2x matings in potatoes

  • A. O. Mendiburu
  • S. J. Peloquin


Two of the four strands of a bivalent are recovered together in tetraploid progeny arising from 4x-2x matings. This provides a method for gene and centromere mapping. The cross pppp x Pp produced 62 nulliplex tetraploid individuals in a total of 951, i.e., 6.5%. The diploid clone was found to produce diplandrous gametes through first division restitution (FDR). The map distance P-centromere was estimated as 13.0 units, the limiting values at the 95% binomial confidence interval being 10.1 and 16.3. The mapping method is explained in detail and a formula is deduced to obtain genotypic series of 2n gametes under particular assumptions. The direction 4x×2x is advantageous, since high seed set diploid clones which give 99% tetraploid progeny, and whose method of diplandroid formation is known are already available. Diploid clones heterozygous for many markers and tetraploids nulliplex for the same markers are needed to fully exploit this method.

Key words

Half-tetrad analysis 2n gametes Potato Gene-centromere mapping 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, E.G.: Crossing over in a case of attached x chromosomes in Drosophila melanogaster. Genetics 10, 403–417 (1925)Google Scholar
  2. Baldwin, M.; Chovnick, A.: Autosomal half-tetrad analysis in Drosophila melanogaster. Genetics 55, 277–293 (1967)Google Scholar
  3. Beadle, G.W.; Emerson, S.: Further studies of crossing over in attached x chromosomes of Drosophila melanogaster. Genetics 20, 192–206 (1935)Google Scholar
  4. Crow, J.F.: Genetics and medicine. In: Heritage from Mendel. Madison Milwaukee London: University of Wisconsin Press 1967Google Scholar
  5. De Jong, H.; Rowe, P.R.: Inbreeding in cultivated diploid potatoes. Potato Res. 14, 74–83 (1971)Google Scholar
  6. Hanneman, R.E.; Peloquin, S.J.: Ploidy levels of progeny from diploid-tetraploid crosses in the potato. Amer. Potato J. 45, 255–261 (1968)Google Scholar
  7. Howard, H.W.: Genetics of the potato, Solanum tuberosum. Berlin-Heidelberg-New York: Springer 1970Google Scholar
  8. Lindsley, D.L.; Frankhauser, G.; Humphrey, R.R.: Mapping centromeres in the axolotl. Genetics 41, 58–64 (1956)Google Scholar
  9. Lunden, A.P.: Arvelighetsundersokelser i potet. Meld. Norges Landbruk. 20, 1–159 (1937)Google Scholar
  10. Mainland, D.; Herrera, L.; Sutcliffe, M.L.: Statistical tables for use with binomial samples. Dept. of Medical Statistics. New York: University College of Medicine, pp. 83 1956Google Scholar
  11. Mok, D.W.S.; Peloquin, S.J.: Three mechanisms of 2n pollen formation in diploid potatoes. Can. J. Genet. Cytol. 17, 217–225 (1975)Google Scholar
  12. Rhoades, M.M.; Dempsey, Ellen: Induction of chromosome doubling at meiosis by the elongate gene in maize. Genetics 54, 505–522 (1966)Google Scholar
  13. Volpe, E.P.: Chromosome mapping in the leopard frog. Genetics 64, 11–21 (1970)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • A. O. Mendiburu
    • 1
    • 2
  • S. J. Peloquin
    • 1
  1. 1.Department of Genetics and HorticultureUniversity of WisconsinMadisonUSA
  2. 2.Department of Plant ProductionEERA, INTABalcarceArgentina

Personalised recommendations