Archives of Microbiology

, Volume 156, Issue 4, pp 312–318

Cell wall and sheath constituents of the cyanobacterium Gloeobacter violaceus

  • Sabine Schneider
  • Uwe J. Jürgens
Original Papers
  • 177 Downloads

Abstract

Sheaths isolated from Gloeobacter violaceus were found to be composed of a major polysaccharide moiety (glucose, galactose, rhamnose, mannose, arabinose), a protein moiety, and negatively charged components (glucuronic acids, phosphate, sulfate). Outer membrane polypeptide patterns were dominated by two major peptidoglycan-associated proteins (Mr 62,000 and 53,000). Lipopolysaccharide constituents were glucosamine, 3-hydroxy fatty acids (3-OH-14:0, anteiso-3-OH-15:0, 3-OH-16:0, 3-OH-18:0), carbohydrates, and phosphate. A1γ-type peptidoglycan and non-peptidoglycan components (mannosamine, glucose, mannose, and glucosamine) indicated the presence of a peptidoglycan-polysaccharide complex in the cell walls of Gloeobacter violaceus.

Key words

Cell wall Cyanobacterium Gloeobacter violaceus Lipopolysaccharide Outer membrane protein Peptidoglycan-polysaccharide complex Sheath 

Abbreviations

A2pm

diaminopimelic acid

ATCC

American Type Culture Collection

CE

cell envelope

CM

cytoplasmic membrane

CW

cell wall

dOcla

3-deoxy-d-manno-2-octulosonic acid

GalN

galactosamine

GlcN

glucosamine

GlcUA

glucuronic acid

HF

hydrofluoric acid

LPS

lipopolysaccharide

ManN

mannosamine

M

relative molecular mass

MurN

muramic acid

MurN-6-P

muramic acid-6-phosphate

OMe

O-methyl

PAGE

polyacrylamide gel electrophoresis

PCC

Pasteur Culture Collection

SDS

sodium dodecyl sulfate

SH

sheath

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Sabine Schneider
    • 1
  • Uwe J. Jürgens
    • 1
  1. 1.Institut für Biologie II, MikrobiologieAlbert-Ludwigs-UniversitätFreiburg i. Br.Federal Republic of Germany

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