Archives of Microbiology

, Volume 156, Issue 4, pp 239–247

Methanopyrus kandleri, gen. and sp. nov. represents a novel group of hyperthermophilic methanogens, growing at 110°C

  • Margit Kurr
  • Robert Huber
  • Helmut König
  • Holger W. Jannasch
  • Hans Fricke
  • Antonio Trincone
  • Jakob K. Kristjansson
  • Karl O. Stetter
Original Papers

Abstract

A novel group of hyperthermophilic rod-shaped motile methanogens was isolated from a hydrothermally heated deep sea sediment (Guaymas Basin, Gulf of California) and from a shallow marine hydrothermal system (Kolbeinsey ridge, Iceland). The grew between 84 and 110°C (opt: 98°C) and from 0.2% to 4% NaCl (opt. 2%) and pH 5.5 to 7 (opt: 6.5). The isolates were obligate chemolithoautotrophes using H2/CO2 as energy and carbon sources. In the presence of sulfur, H2S was formed and cells tended to lyse. The cell wall consisted of a new type of pseudomurein containing ornithin in addition to lysine and no N-acetylglucosamine. The pseudomurein layer was covered by a detergent-sensitive protein surface layer. The core lipid consisted exclusively of phytanyl diether. The GC content of the DNA was 60 mol%. By 16S rRNA comparisons the new organisms were not related to any of the three methanogenic lineages. Based on the physiological and molecular properties of the new isolates, we describe here a new genus, which we name Methanopyrus (the “methane fire”). The type species is Methanopyrus kandleri (type strain: AV19; DSM 6324).

Key words

Methanopyrus Methanogens Archaea Hyperthermophilic Marine Vents 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Margit Kurr
    • 1
  • Robert Huber
    • 1
  • Helmut König
    • 2
  • Holger W. Jannasch
    • 3
  • Hans Fricke
    • 4
  • Antonio Trincone
    • 5
  • Jakob K. Kristjansson
    • 6
  • Karl O. Stetter
    • 1
  1. 1.Lehrstuhl für MikrobiologieUniversität RegensburgRegensburgFederal Republic of Germany
  2. 2.Abteilung Angewandte MikrobiologieUniversität UlmUlmFederal Republic of Germany
  3. 3.Department of BiologyWoods Hole Oceanographic InstitutionWoods HoleUSA
  4. 4.Max Planck-Institut für VerhaltensphysiologieSeewiesenFederal Republic of Germany
  5. 5.Istituto per la Chimica di Molecole di Interesse BiologicoArco Felice (Napoli)Italy
  6. 6.Institute of BiologyUniversity of IcelandReykjavikIceland

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