Theoretical and Applied Genetics

, Volume 69, Issue 1, pp 55–61 | Cite as

Diallel analysis of androgenetic plant production in hexaploid Triticale (X. triticosecale, Wittmack)

  • G. Charmet
  • S. Bernard


Studies were made on the genetic determination of androgenetic plant yield and its two components: embryo production and green plant regeneration. This involved the analysis of a complete 7×7 diallel cross of 4 androgenetic lines and 3 lines obtained by pedigree selection, one of them having the Triticum timopheevi cytoplasm. The three traits analysed are both heritable and environmentally influenced (by season and culture medium composition). The analysis of embryo production shows a mainly nuclear inheritance, with predominantly additive gene action, but also a favourable effect of Triticum timopheevi cytoplasm. Green plant regeneration has a more complex genetic determination, with additive as well as non-additive gene action and cytoplasmic influences. Hybrids appear superior to inbred lines for embryogenesis and green plant yield, but not for green plant regeneration. Androgenetic lines used as parents did not show superiority over other parents either in their own value or in the transmission of androgenetic abilities. Genetic improvement seems to be possible by recombination in progenies of hybrids between lines having complementary abilities.

Key words

Hexaploid Triticale Anther culture Androgenetic responsiveness Diallel analysis Genetic control 


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  1. Barclay IR (1975) High frequencies of haploid production in wheat (Triticum aestivum) by chromosome elimination. Nature 256:410–411Google Scholar
  2. Bernard S (1977) Etude de quelques facteurs contribuant à la réussite de l'androgénèse par culture d'anthères in vitro chez le triticale hexaploide. Ann Amelior Plant 27:639–655Google Scholar
  3. Bernard S (1980) In vitro androgenesis in hexaploid triticale: determination of physical conditions increasing embryoid and green plant production. Z Pflanzenzücht 85:308–321Google Scholar
  4. Buiatti M, Baroncelli S, Bennici A, Pagliai M, Tesi R (1974) Genetic of differentiation in vitro of Brassica oleracea var. Botrytis. II and in vitro and in vivo analysis of diallel cross. Z Pflanzenzücht 72:269–274Google Scholar
  5. Bullock WP, Baenziger PS, Schaeffer GW, Bottino PJ (1982) Anther culture of wheat (Triticum aestivum L.) F1's and their reciprocal crosses. Theor Appl Genet 62:155–159Google Scholar
  6. De Buyser J, Henry Y, Laur R, Lonnet P (1981) Utilisation de l'androgénèse in vitro dans les programmes de sélection du blé tendre (Triticum aestivum L.). Z Pflanzenzüucht 87:290–299Google Scholar
  7. Falk DE, Kasha KJ (1983) Genetic studies of the crossibility of hexaploid wheat with rye and Hordeum bulbosum. Theor Appl Genet 64:303–307Google Scholar
  8. Foroughi-Wehr B, Friedt W, Wenzel G (1982) On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor Appl Genet 62:233–239Google Scholar
  9. Foroughi-Wehr B, Friedt W (1984) Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor Appl Genet 67:377–382Google Scholar
  10. Friedt W, Foroughi-Wehr B (1983) Field performance of Androgenetic doubled haploid spring barley from F1 hybrids. Z Pflanzenzücht 90:177–184Google Scholar
  11. Gallais A (1978) Place de l'haploïdie dans un schéma de sélection. Sélectionneur Franç 26:39–49Google Scholar
  12. Griffing B (1956) Concept of general and specific combining ability in relation to diallel crossing systems. Aust J Biol Sci 9:463–493Google Scholar
  13. Griffing B (1975) Efficiency changes due to use of doubled haploids in recurrent selection methods. Theor Appl Genet 46:367–386Google Scholar
  14. Henry Y, De Buyser J (1981) Float culture of wheat anthers. Theor Appl Genet 60:77–79Google Scholar
  15. Huffman F, Thomas E, Wenzel G (1982) Anther culture as a breeding tool in rape. 2. progeny analyses of androgenetic lines and induced mutants from haploid cultures. Theor Appl Genet 61:225–232Google Scholar
  16. Kao FN (1981) Plant formation from Barley anther culture with Ficoll Media. Z Pflanzenphysiol 103:437–443Google Scholar
  17. Kasha RJ, Reinbergs E (1980) Achievements with haploids in barley research and breeding. In: Davies DB, Hopwood DA (eds) The plant genome. Norwich, pp 215–230Google Scholar
  18. Lange W (1971) Crosses between Hordeum vulgare and H. bulbosum. 2. Elimination of chromosomes in hybrid tissues. Euphytica 20:181–194Google Scholar
  19. Maheshwari SC, Tyagi AK, Malhotra K, Sopory SK (1980) Induction of haploidy from pollen grains in angiosperms — the current status. Theor Appl Genet 58:193–206Google Scholar
  20. Nesticky M, Novak FJ, Piovarci A, Dolezeloua M (1983) Genetic analysis of callus growth of maize (Zea mays L.) in vitro. Z Pflanzenzücht 91:322–328Google Scholar
  21. Ouyang JW, Zhou SM, Jia SE (1983) The response of anther culture to culture temperature in Triticum aestivum. Theor Appl Genet 66:101–109Google Scholar
  22. Picard E, De Buyser J (1977) High production of embryoids in anther culture of pollen derived homozygous spring wheat. Ann Amelior Plant 27:483–488Google Scholar
  23. Picard E, De Buyser J, Henry Y (1978) Techniques de production d'haploïdes de blé par culture d'anthères in vitro. Sélectionneur Franç 26:25–37Google Scholar
  24. Research Group 301 (1976) A sharp increase of the frequency of pollen plant induction in wheat with potato medium. Acta Genet Sinica 3:25–31Google Scholar
  25. Sozinov A, Lukjanjuk S, Ignatova S (1981) Anther cultivation and induction of haploid plants in triticale. Z Pflanzenzücht 86:272–285Google Scholar
  26. Wenzel G, Hoffmann F, Thomas E (1977) Increased induction and chromosome doubling of androgenetic haploid rye. Theor Appl Genet 51:81–86Google Scholar
  27. Wenzel G, Uhrig H (1981) Breeding for nematode and virus resistance in potato via anther culture. Theor Appl Genet 59:333–340Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • G. Charmet
    • 1
  • S. Bernard
    • 1
  1. 1.Institut National de la Recherche AgronomiqueStation d'Amélioration des PlantesClermont-Ferrand, CedexFrance

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