Theoretical and Applied Genetics

, Volume 69, Issue 1, pp 47–53 | Cite as

Enhanced available methionine concentration associated with higher phaseolin levels in common bean seeds

  • P. Gepts
  • F. A. Bliss
Article

Summary

The relationship between available methionine concentration and the levels of phaseolin — the major seed storage proteins of the common bean — was studied using three groups of genetic materials: First, the F2 progenies of interspecific crosses between P. vulgaris cultivars and aP. coccineus subsp. coccineus line (cv. ‘Mexican Red Runner’) having no detectable phaseolin; second, the F2 progenies and segregating F3 families of crosses between cultivated P. vulgaris lines and a Mexican wild bean accession (PI 325690-3) carrying a gene producing a reduction in phaseolin content; third, two inbred backcross populations: ‘Sanilac’x‘Bush Blue Lake 240’ (population 2) and ‘Sanilac’x‘15R 148’ (population 6). Total seed N levels were determined by micro-Kjeldahl, phaseolin levels by rocket immunoelectrophoresis and available methionine levels by the Streptococcus zymogenes bioassay. Our results indicate that in all the genetic materials studied, with the exception of population 6, higher phaseolin levels lead to increased available methionine concentration. Although phaseolin has a low methionine concentration, it is actually a major source of available methionine in common bean seeds, because it represents a large part of total seed nitrogen and because limited differences exist between the methionine concentrations of the different protein fractions. This contrasts with the situation in cereals such as maize, barley and sorghum, where increased levels of the major limiting amino acid (lysine) can be achieved through a decrease in the amounts of the main seed storage protein fraction (prolamines). In population 6, no relationship was observed between available methionine and phaseolin content. Other factors, such as additional methionine-rich polypeptides or the presence of tannins, might obscure the positive relationship between phaseolin and available methionine content in population 6.

Key words

Phaseolus vulgaris L. Streptococcus zymogenes bioassay Rocket immunoelectrophoresis Micro-Kjeldahl Limiting essential amino acid 

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References

  1. Association of Official Agricultural Chemists (1960) Official methods of analysis, 9th edn. The Association, Washington DCGoogle Scholar
  2. Blake T, Bliss FA (1983) Purification and characterization of a novel major bean seed protein. Agron Abstr ASA-CSSA-SSSA Annual Meetings, 1983Google Scholar
  3. Bliss FA, Brown JWS (1983) Breeding common bean for improved quantity and quality of seed proteins. In: Janick J (ed) Plant Breeding reviews, vol 1. AVI, Westport Conn, USA, pp 59–102Google Scholar
  4. Bressani R (1973) Legumes in human diets and how they might be improved. In: Mimer M (ed) Nutritional improvement of food legumes by breeding. PAG, United Nations, New York, USA, pp 15–42Google Scholar
  5. Bressani R, Elias LG (1980) The nutritional role of polyphenols in beans. In: Hulse JH (ed) Polyphenols in cereals and legumes. International Development Research Centre, Ottawa, Canada, pp 61–72Google Scholar
  6. Bright SWJ, Shewry PR (1983) Improvement of protein quality in cereals. CRC Critical Rev Plant Sci 1:49–93Google Scholar
  7. FAO (1980) Food balance sheets: 1975–1977 average. Food and Agricultural Organization, RomeGoogle Scholar
  8. Fernandez F, Gepts P, Lopez M (1983) Etapas de desarrollo de la planta de frijol comun (Phaseolus vulgaris). CIAT, Cali, Colombia, Series 045B-09.03Google Scholar
  9. Guiragossian V, Chibber BAK, Van Scoyoc S, Jambunatham R, Mertz ET, Axteil JD (1978) Characteristics of proteins from normal, high lysine, and high tannin sorghums. J Agric Food Chem 26:219–223Google Scholar
  10. Kelly JD, Bliss FA (1975) Quality factors affecting the nutritive value of bean seed proteins. Crop Sci 15:757–760Google Scholar
  11. Kelly JF (1973) Increasing protein quantity and quality. In: Milner M (ed) Nutritional improvement of food legumes by breeding. PAG, United Nations, New York, USA, pp 179–184Google Scholar
  12. Ma Y, Bliss FA (1978) Seed proteins of common bean. Crop Sci 17:431–437Google Scholar
  13. Mertz ET, Bates LS, Nelson OE (1964) Mutant gene that changes protein composition and increases lysine content in maize endosperm. Science 145:279–280Google Scholar
  14. Miflin BJ, Shewry PR (1979) The synthesis of proteins in normal and high lysine barley seed. In: Laidman DL, Wyn Jones RG (eds) Recent advances in the biochemistry of cereals. Academic Press, London New York, pp 239–273Google Scholar
  15. Misra PS, Mertz ET, Glover DV (1975) Studies on corn proteins. 6. Endosperm protein changes in single and double endosperm mutants of maize. Cereal Chem 52:161–166Google Scholar
  16. Mutschler MA, Bliss FA (1981) Inheritance of bean seed globulin content and its relationship to protein content and quality. Crop Sci 21:289–294Google Scholar
  17. Nelson OE (1980) Genetic control of polysaccharide and storage protein synthesis in the endosperms of barley, maize, and sorghum. In: Pomeranz Y (ed) Advances in cereal science and technology, vol III. Am Assoc Cereal Chem, St Paul Minn, pp 41–71Google Scholar
  18. Romero-Andreas J, Sun SM, McLeester RC, Bliss FA, Hall TC (1975) Heritable variation in a polypeptide subunit of the major storage protein of the bean (Phaseolus vulgaris L.). Plant Physiol 56:776–779Google Scholar
  19. Romero-Andreas J, Bliss FA (1982) A dominant regulator gene of phaseolin. HortScience 17:504Google Scholar
  20. Shewry PR, Faulks AJ, Miflin BJ (1980) Effect of high-lysine mutations on the protein fractions of barley grain. Biochem Genet 18:133–151Google Scholar
  21. Shewry PR, Pratt HM, Leggatt MM, Miflin BJ (1979) Protein metabolism in developing endosperms of high-lysine and normal barley. Cereal Chem 56:110–117Google Scholar
  22. Singh R, Axtell JD (1973) High lysine mutant gene (hl) that improves protein quality and biological value of grain sorghum. Crop Sci 535–539Google Scholar
  23. Sodek L, Wilson CM (1971) Amino acid composition of proteins isolated from normal, opaque-2 and floury-2 endosperms by a modified Osborne procedure. J Agric Food Chem 19:1144–1150Google Scholar
  24. Sullivan J, Bliss FA (1983) Genetic control of quantitative variation in phaseolin seed protein of common bean. J Am Soc Hortic Sci 108:782–787Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • P. Gepts
    • 1
  • F. A. Bliss
    • 1
  1. 1.Department of HorticultureUniversity of WisconsinMadisonUSA

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