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Molecular and General Genetics MGG

, Volume 224, Issue 3, pp 347–356 | Cite as

Direct screening of a small genome : Estimation of the magnitude of plant gene expression changes during adaptation to high salt

  • Gabriele Meyer
  • Jürgen M. Schmitt
  • Hans J. Bohnert
Article

Summary

Mesembryanthemum crystallinum (common ice plant), a facultative halophyte with a genome size of 393 000 kb, was used to estimate the magnitude of changes in gene expression in response to environmental stress by excess salt. Such treatment induces a water-conserving pathway of carbon assimilation (CAM) which is, at least in part, transcriptionally controlled. From a genomic library, 200 phage containing approximately 3200 kb (0.8% of the genome) were randomly selected. The inserts in these clones could be divided into four classes ranging from highly repetitive DNA (class I clones) to single-copy DNA (class IV clones). The inserts of the 166 clones of classes II to IV were digested with various restriction enzymes and the fragments were analyzed by hybridization with radioactively labelled mRNA isolated from stressed and unstressed leaves. We found that a total of ∼ 140 DNA fragments hybridized with the RNA probe. Among those, several differentially regulated transcripts were observed. Stress-dependent fluctuation of mRNA abundance was verified by Northern analyses: one mRNA, not detectable in unstressed leaves, appeared in stressed leaves, while steady-state levels of three transcripts decreased during stress. All regulated signals are derived from low abundance mRNAs, which may be missed during screening of cDNA libraries. We conclude from these results that, for the entire genome, on the order of more than one hundred genes are differentially regulated in response to salt stress.

Key words

Genome organization Environmental stress Changes in gene expression Mesembryanthemum crystallinum 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Gabriele Meyer
    • 1
  • Jürgen M. Schmitt
    • 2
  • Hans J. Bohnert
    • 1
    • 3
  1. 1.Department of BiochemistryThe University of ArizonaTucsonUSA
  2. 2.Botanisches InstitutUnversität WürzburgWürzburgGermany
  3. 3.Departments of Molecular & Cellular Biology and of Plant SciencesThe University of ArizonaTucsonUSA

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