Advertisement

Cell and Tissue Research

, Volume 257, Issue 2, pp 279–284 | Cite as

Changes in endoplasmic reticulum during spermiogenesis in the mouse

  • Tadashi Nakamoto
  • Yasuhiro Sakai
Article

Summary

Changes in the endoplasmic reticulum of mouse spermatids during spermiogenesis were examined by scanning electron microscopy, applying the OsO4-DMSO-OsO4 method, which permits 3-dimensional observation of cell organelles. At the same time, the endoplasmic reticulum was stained selectively by the Ur-Pb-Cu method, and 0.5 μm-thick sections were prepared for observation by transmission electron microscopy. The results demonstrated stereoscopically the mode of disappearance of the endoplasmic reticulum. In spermatids of the early maturation phase, the endoplasmic reticulum was of uniform diameter, branched and anastomosed, forming a complicated three-dimensional network throughout the cytoplasm. A two-dimensional net was also noted to have formed just beneath the plasma membrane and about Sertoli cell processes invaginating the spermatid cytoplasm. As spermiogenesis progressed, the spread-out endoplasmic reticulum gradually aggregated to form a condensed, glomerulus-like structure consisting of a very thin endoplasmic reticulum connected to the surrounding endoplasmic reticulum. This structure corresponds to the so-called “radial body”. Thus, the endoplasmic reticulum may aggregate, condense, be transformed into a radial body, and be removed from the cytoplasm. The two-dimensional endoplasmic reticulum-net, just beneath the plasma membrane and surrounding processes of Sertoli cells, disappeared in spaces where the three-dimensional endoplasmic reticulum network was scarce. Both the two-dimensional endoplasmic reticulum-net structure and the three-dimensional endoplasmic reticulum network disappeared at the same time, indicating that they may be closely related.

Key words

Spermiogenesis Endoplasmic reticulum Radial body Ur-Pb-Cu method Scanning electron microscopy (SEM) Mouse (BALB/c) 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Christensen AK (1965) The fine structure of testicular interstitial cells in guinea pigs. J Cell Biol 26:911–935Google Scholar
  2. Clermont Y, Rambourg A (1978) Evolution of the endoplasmic reticulum during rat spermiogenesis. Am J Anat 151:191–212Google Scholar
  3. Dooher GB, Bennett D (1973) Fine structural observations on the development of the sperm head in the mouse. Am J Anat 136:339–362Google Scholar
  4. Inoue T (1982) The continuity of endoplasmic reticulum in rat spermatids observed by scanning electron microscopy. J Electron Microsc (Tokyo) 31:261–263Google Scholar
  5. Morales C, Clermont Y (1982) Evolution of Sertoli cell processes invading the cytoplasm of rat spermatids. Anat Rec 203:233–244Google Scholar
  6. Oakberg EF (1956) A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Am J Anat 99:391–413Google Scholar
  7. Palade GE, Porter KR (1954) Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med 100:641–656Google Scholar
  8. Sakai Y, Nakamoto T, Yamashina S (1988) Dynamic changes in Sertoli cell processes invading spermatid cytoplasm during mouse spermiogenesis. Anat Rec 220:51–57Google Scholar
  9. Tanaka K (1980) Scanning electron microscopy of intracellular structures. Int Rev Cytol 68:97–125Google Scholar
  10. Tanaka K, Naguro T (1981) High resolution scanning electron microscopy of cell organelles by a new specimen preparation method. Biomed Res 2, [Suppl]: 63–70Google Scholar
  11. Thiery G, Rambourg A (1976) A new staining technique for studying thick sections in the electron microscope. J Microse Biol Cell 26:103–106Google Scholar
  12. Vogl AW, Soucy LJ, Foo V (1985) Ultrastructure of Sertoli-cell penetrating processes found in germ cells of the golden-mantled ground squirrel (Spermophilus lateralis). Am J Anat 172:75–86Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Tadashi Nakamoto
    • 1
  • Yasuhiro Sakai
    • 1
  1. 1.Department of Anatomy, School of MedicineKitasato UniversityKanagawaJapan

Personalised recommendations