Cytoplasmic effects on the tissue culture response of wheat (Triticum aestivum) callus
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Summary
Calli were initiated from immature embryos of eight lines of hexaploid wheat (Triticum aestivum L. em. Thell) with different cytoplasms, the euplasmic nuclear donor ‘Chinese Spring’ and seven alloplasmic lines derived from wild relative species of the genera Triticum and Aegilops. The calli were found to differ in their initial growth rates, their sensitivity to 2,4-D and their ability to organise shoot primordia, demonstrating that the cytoplasm can significantly affect the behaviour of tissues in culture. The potential for improving the responses of tissues in culture by cytoplasmic changes is noted.
Key words
Cytoplasm Wheat Tissue culture CallusPreview
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References
- Gracen VE, Grogan CD (1974) Diversity and suitability for hybrid production of different sources of cytoplasmic malesterility in maize. Agron J 65:654–657Google Scholar
- Green CE, Phillips RL (1975) Plant regeneration from tissue cultures of maize. Crop Sci 15:417–421Google Scholar
- Kihara H (1951) Substitution of nucleus and its effect on genome manifestations. Cytologia 19:177–193Google Scholar
- Kihara H, Tsunewaki K (1962) Use of an alien cytoplasm as a new method of producing haploids. Jpn J Genet 37:310 to 313Google Scholar
- Maan SS (1979) Specificity of nucleo-cytoplasmic interactions in Triticum and Aegilops species (a review). Wheat Inf Serv 50:71–79Google Scholar
- Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34:915–926Google Scholar
- Matzinger DF, Burk LG (1984) Cytoplasmic modification by anther culture in Nicotiana tabacum L. J Hered 75:167 to170Google Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
- Ogihara Y, Tsunewaki K (1982) Molecular basis of the genetic diversity of the cytoplasm in Triticum and Aegilops. 1. Diversity of the chloroplast genome and its lineage revealed by the restriction pattern of ct-DNAs. Jpn J Genet 57:371–396Google Scholar
- Sears RG, Deckard EL (1982) Tissue culture variability in wheat; callus induction and regeneration. Crop Sci 22:546 to 550Google Scholar
- Shimada T (1978) Plant regeneration from the callus induced from wheat embryos. Jpn J Genet 53:371–374Google Scholar
- Tsunewaki K (1982) Monosomic analysis on the fertility restoration by Triticum aestivum cv ‘Chinese Spring’ against Aegilops ovata cytoplasm. Jpn J Genet 57:517–525Google Scholar
- Tsunewaki K, Mukai Y, Ryu Endo T, Tsuji S, Murata M (1976) Genetic diversity of the cytoplasm in Triticum and Aegilops. 5. Classification of 23 cytoplasms into eight plasma types. Jpn J Genet 51:175–191Google Scholar
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