Theoretical and Applied Genetics

, Volume 77, Issue 5, pp 689–700 | Cite as

The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat

  • O. D. Anderson
  • F. C. Greene
Article

Summary

Two high-molecular-weight subunit (HMWS) glutenin genes from the A and B genomes of the hexaploid bread wheat Triticum aestivum L. cv Cheyenne have been isolated and sequenced. Both of these genes are of the high Mr class (x-type) of HMW glutenins, and have not been previously reported. The entire set of six HMW genes from cultivar Cheyenne have now been isolated and characterized. An analysis of the Ax and Bx sequences shows that the Ax sequence is similar to the homoeologous gene from the D genome, while the Bx repeat structure is significantly different. The repetitive region of these proteins can be modelled as a series of interspersed copies of repeat modifs of 6, 9, and 15 amino acid residues. The evolution of these genes includes single-base substitutions over the entire coding region, plus insertion/deletions of single or blocks of repeats in the central repetitive domain.

Key words

Triticum Homoeoalleles Evolution Dough Gene library 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson OD, Litts JC, Gautier M-F, Greene FC (1984) Nucleic acid sequence and chromosome assignment of a wheat storage protein gene. Nucleic Acid Res 12:8129–8144Google Scholar
  2. Anderson OD, Greene FC, Yip RE, Halford NG, Shewry PR, Malpica-Romero J-M (1989) Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17:461–462Google Scholar
  3. Bartels D, Altosaar I, Harberd NP, Barker RF, Thompson RD (1986) Molecular analysis of γ-gliadin gene families at the complex locus of bread wheat (T. aestivum L.). Theor Appl Genet 72:845–853Google Scholar
  4. Branlard G, Dardevet M (1985) Diversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354Google Scholar
  5. Colot V, Robert LS, Kavanagh TA, Bevan MW, Thompson RD (1987) Localization of sequences in wheat endosperm protein genes which confer tissue-specific expression in tobacco. EMBO J 6:3559–3564Google Scholar
  6. Dale RMK, McClure BA, Houchins JP (1985) A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA. Plasmid 13:31–40Google Scholar
  7. Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J (1986) mRNA Transcripts of several plant genes are polyadenylated at multiple sites in vivo. Nucleic Acids Res 14:2229–2240Google Scholar
  8. Ewart JAD (1968) A hypothesis for the structure and rheology of glutenin. J Sci Food Agric 19:617–623Google Scholar
  9. Forde J, Forde BG, Fry RP, Kreis M, Shewry PR, Miflin BJ (1983) Identification of barley and wheat cDNA clones related to the high-Mr polypeptides of wheat gluten. FEBS Lett 162:360–366Google Scholar
  10. Forde J, Malpica J-M, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Res 13:6817–6832Google Scholar
  11. Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842Google Scholar
  12. Galili G, Feldman M (1983) Genetic control of endosperm proteins in wheat: 2. Variation in high molecular weight glutenin and gliadin subunits of Triticum aestivum. Theor Appl Genet 66:77–86Google Scholar
  13. Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126Google Scholar
  14. Harberd NP, Bartels D, Thompson RD (1986) DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits in wheat. Biochem Genet 24:579–596Google Scholar
  15. Harberd NP, Flavell RB, Thompson RD (1987) Identification of a transposon-like insertion in a Glu-1 allele of wheat. Mol Gen Genet 209:326–332Google Scholar
  16. Kozak M (1987) An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acid Res 15:8125–8148Google Scholar
  17. Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those for wheat, barely and rye. Oxford Surv Plant Mol Cell Biol 2:253–317Google Scholar
  18. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NYGoogle Scholar
  19. Mead DA, Szczesna-Skorupa E, Kemper B (1986) Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng 1:67–74Google Scholar
  20. Messing J, Crea R, Seeburg PH (1981) A system for shotgun DNA sequencing. Nucleic Acids Res 9:309–321Google Scholar
  21. Payne PI, Harris PA, Law CN, Holt LM, Blackman JA (1980) The high-molecular-weight subunits of glutenin: structure, genetics and relationship to bread-making quality. Ann Technol Agric 29:309–320Google Scholar
  22. Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 1: allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236Google Scholar
  23. Payne PI, Holt LM, Worland AG, Law CN (1982) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 3: telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138Google Scholar
  24. Payne PI, Holt LM, Thompson RD, Bartels D, Harberd NP, Harris PA, Law CN (1983) The high-molecular-weight glutenins: classical genetics, molecular genetics and the relationship to bread-making quality. Proc 6th Int Wheat Genet Symp 827–834Google Scholar
  25. Payne PI, Holt LM, Jackson EA, Law CN (1984) Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Philos Trans R Soc London Ser B 304:359–371Google Scholar
  26. Sanchez-Pescador R, Urdea MS (1984) Use of unpurified primers in sequencing. DNA 3:339–343Google Scholar
  27. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. PNAS 74:5463–5467Google Scholar
  28. Shewry PR, Field JM, Faulks AJ, Parmar S, Miflin BJ, Dietler MD, Lew E, Kasarda DD (1984) The purification and n-terminal amino acid sequence analysis of the high molecular weight gluten polypeptides of wheat. Biochim Biophy Acta 788:23–34Google Scholar
  29. Sugiyama T, Rafalski A, Peterson D, Söll D (1985) A wheat HMW glutenin gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8737Google Scholar
  30. Tatham AS, Shewry PR, Miflin BJ (1984) Wheat gluten elasticity: a similar molecular basis to elastin? FEBS Lett 177:205–208Google Scholar
  31. Thompson RD, Bartels D, Harberd NP, Flavell RB (1983) Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones. Theor Appl Genet 67:87–96Google Scholar
  32. Thompson RD, Bartels D, Harberd NP (1985) Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 13:6833–6846Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • O. D. Anderson
    • 1
  • F. C. Greene
    • 1
  1. 1.USDA, ARS, Western Regional Research CenterAlbanyUSA

Personalised recommendations