Molecular and General Genetics MGG

, Volume 219, Issue 1–2, pp 125–128

Mutations in the genes for mitochondrial RNA polymerase and a second mitochondrial transcription factor of Saccharomyces cerevisiae

  • Thomas Lisowsky
  • Georg Michaelis


In our previous work (Lisowsky et al. 1987; Lisowsky and Michaelis 1988) we have identified two nuclear pet genes of yeast that are required for mitochondrial transcription. In this report we show that one of these pet mutations, pet-ts798, maps in the RP041 gene encoding mitochondrial RNA polymerase. The temperature-sensitive lesion of mutant pet-ts798 can be suppressed by a second nuclear gene RF1023 (mtf1) when inserted into a high copy number plasmid. Our assumption that mtf1 codes for a 40 kDa mitochondrial transcription factor is supported by the fact that the cloned gene acts as an intergenic suppressor of a temperature-sensitive RNA polymerase mutant. A third nuclear gene (mtf2) for mitochondrial transcription was identified by analysing mutant pet-ts3504. The in vitro transcriptional activity of isolated mutant mitochondria is temperature sensitive suggesting the presence of an altered component of transcription inside mitochondria. The defect was confirmed by studies with a transcriptionally active DNA-protein complex and by testing the DNA-binding ability of mitochondrial proteins.

Key words

Yeast Saccharomyces cerevisiae Nuclear pet genes Mitochondrial RNA polymerase Mitochondrial transcription Two transcription factors 


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  1. Broach JR, Strathern JN, Hicks JB (1979) Transformation in yeast: Development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8:121–133Google Scholar
  2. Faye G, Kujawa C, Fukuhara H (1974) Physical and genetic organization of petite and grande yeast mitochondrial DNA. J Mol Biol 88:185–203Google Scholar
  3. Greenleaf AL, Kelly JL, Lehman IR (1986) Yeast RP041 gene product is required for transcription and maintenance of the mitochondrial genome. Proc Natl Acad Sci USA 83:3391–3394Google Scholar
  4. Kelly JL, Lehman IR (1986) Yeast mitochondrial RNA polymerase. J Biol Chem 261:10340–10347Google Scholar
  5. Kelly JL, Greenleaf AL, Lehman IR (1986) Isolation of the nuclear gene coding a subunit of the yeast mitochondrial RNA polymerase. J Biol Chem 261:10348–10351Google Scholar
  6. Lisowsky T (1987) Die in vitro Analyse der mitochondrialen Transkription von Mutanten der Hefe, Saccharomyces cerevisiae: Isolation und Charakterisierung eines nukleären Genes. Thesis, University of DüsseldorfGoogle Scholar
  7. Lisowsky T, Michaelis G (1988) A nuclear gene essential for mitochondrial replication suppresses a defect of mitochondrial transcription in Saccharomyces cerevisiae. Mol Gen Genet 214:218–223Google Scholar
  8. Lisowsky T, Schweizer E, Michaelis G (1987) A nuclear mutation affecting mitochondrial transcription in Saccharomyces cerevisiae. Eur J Biochem 164:559–563Google Scholar
  9. Ma J, Ptashne M (1987) A new class of yeast transcriptional activators. Cell 51:113–119Google Scholar
  10. Masters BS, Stohl LL, Clayton DA (1987) Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7. Cell 51:89–99CrossRefPubMedGoogle Scholar
  11. Michaelis G, Mannhaupt G, Pratje E, Fischer E, Naggert J, Schweizer E (1982) Mitochondrial translation products in nuclear respiration-deficient pet mutants of Saccharomyces cerevisiae. In: Slonimski PP, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 311–321Google Scholar
  12. Osinga KA, Evers RF, Laan JC vaan der, Tabak HF (1981) A putative precursor for the small ribosomal RNA from mitochondria. Nucleic Acids Res 9:1351–1364Google Scholar
  13. Schinkel AH, Groot Koerkamp MJA, Touw EPW, Tabak HF (1987) Specificity factor of yeast mitochondrial RNA polymerase. J Biol Chem 262:12785–12791Google Scholar
  14. Schinkel AH, Groot Koerkamp MJA, Tabak HF (1988a) Mitochondrial RNA polymerase of Saccharomyces cerevisiae: Composition and mechanism of promoter recognition. EMBO J 7:3255–3262Google Scholar
  15. Schinkel AH, Groot Koerkamp MJA, Teunissen AWRH, Tabak HF (1988b) RNA polymerase induces DNA bending at yeast mitochondrial promoters. Nucleic Acids Res 16:9147–9163Google Scholar
  16. Ticho BS, Getz GS (1988) The characterization of yeast mitochondrial RNA polymerase. A monomer of 150,000 Daltons with a transcription factor of 70,000 Daltons. J Biol Chem 263:10096–10103Google Scholar
  17. Wilcoxen SE, Peterson CR, Winkley CS, Keller MJ, Jaehning JA (1988) Two forms of RP041-dependent RNA polymerase. J Biol Chem 263:12346–12351Google Scholar
  18. Wolf K, Dujon B, Slonimski PP (1973) Mitochondrial genetics V. Multifactorial mitochondrial crosses involving a mutation conferring paromomycin-resistance in Saccharomyces cerevisiae. Mol Gen Genet 125:53–90Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Thomas Lisowsky
    • 1
  • Georg Michaelis
    • 1
  1. 1.Botanisches InstitutUniversität DüsseldorfDüsseldorfGermany

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