Molecular and General Genetics MGG

, Volume 220, Issue 1, pp 121–126 | Cite as

Novel genes influencing the expression of the yellow locus and mdg4 (gypsy) in Drosophila melanogaster

  • Pavel G. Georgiev
  • Tatiana I. Gerasimova


We used a system with a mobilized Stalker transposable element, sometimes in combination with P-M hybrid dysgenesis, in the search for new mutations interfering with the y2 mutation induced by mdg4 (gypsy) insertion into the yellow locus. A novel gene, modifier of mdg4, was detected in chromosome 3. The mutation mod(mdg4) either enhanced or suppressed phenotypic changes in different mutations induced by mdg4 insertions. Thus, mod(mdg4) seems to be involved in the control of mdg4 expression. Six other loci designated as enhancers of yellow were also detected. The e(y) n (with n from 1–6) mutations enhanced the expression of several y mutations induced by different insertions into the yellow locus. The major change is a damage of bristle and hair pigmentation which is not suppressed by su(Hw) mutations. On the other hand, e(y) n alleles do not interact with mdg4 induced mutations in other loci. All e(y) n genes are located in different regions of the X chromosome. One may speculate that e(y) n genes are involved in trans-regulation of the yellow locus and possibly of some other loci.

Key words

Modifier of mdg4 Enhancers of yellow Stalker Yellow mdg4 (gypsy


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  1. Biessman H (1985) Molecular analysis of the yellow gene (y) region of Drosophila melanogaster. Proc Natl Acad Sci USA 82:7369–7373Google Scholar
  2. Campuzano S, Carramolino L, Cabrera CV, Ruiz Gomez M, Villares R, Boronat A, Modolell J (1985) Molecular genetics of the achete-scute gene complex of D. melanogaster. Cell 40:327–338CrossRefPubMedGoogle Scholar
  3. Chia W, Howes G, Martin M, Meng YB, Moses K, Tsubota S (1986) Molecular analysis of the yellow locus of Drosophila. EMBO J 5:3597–3605Google Scholar
  4. Garcia-Bellido A (1986) Genetic analysis of morphogenesis. In: Gustaffson JP, Stebbins GL, Ayala FJ (eds) Genetics, Development and Evolution. Plenum Publishing Corporation, New York, pp 187–209Google Scholar
  5. Georgiev GP, Tchurikov NA, Ilyin YV, Georgieva SG, Mizrokhi LJ, Priimagi AF, Gerasimova TI, Georgiev PG, Simonova OB, Kiselev SL, Kochieva EZ (1989) Mobile genetic elements in Drosophila melanogaster (recent experiments). Genome, in pressGoogle Scholar
  6. Georgiev PG, Mogila VA, Korochkina SE, Gerasimova TI (1986) Obtaining of Drosophila melanogaster strains with prolonged instability. Proc Acad Sci USSR (in Russian) 291:1492–1495Google Scholar
  7. Geyer PK, Corces VG (1987) Separate regulatory elements are responsible for the complex pattern of tissue-specific and developmental transcription of the yellow locus in Drosophila melanogaster. Genes Dev 1:996–1004Google Scholar
  8. Geyer PK, Spana C, Corces VG (1986) On the molecular mechanism of gypsy induced mutations of the yellow locus of Drosophila melanogaster. EMBO J 5:2657–2662Google Scholar
  9. Green MM (1961) Complementation at the yellow locus in Drosophila melanogaster. Genetics 46:1385–1388Google Scholar
  10. Kidwell MG (1986) P-M mutagenesis. In: Roberts DB (ed) Drosophila: a practical approach. IRL Press, Oxford, pp 59–82Google Scholar
  11. Lindsley DL, Grell EH (1968) Genetic variations of Drosophila melanogaster. Carnegie Inst Wash Publ No 627Google Scholar
  12. Maniatis T, Goodbourn S, Fischer JA (1987) Regulation of inducible and tissue-specific gene expression. Science 236:1237–1244Google Scholar
  13. Mazo AM, Mizrokhi LJ, Karavanov AA, Sedkov YA, Krichevskaya AA, Ilyin YV (1989) Suppression in Drosophila: su(Hw) and su(f) gene products interact with a region of gypsy(mdg4) regulating its transcriptional activity. EMBO J 8:903–911Google Scholar
  14. Modolell J, Bender W, Meselson M (1983) Drosophila melanogaster mutations suppressible by the suppressor of Hairy wing are insertions of 7.3 kb mobile element. Proc Natl Acad Sci USA 8:1678–1681Google Scholar
  15. Nash WG (1976) Patterns of pigmentation color states regulated by the yellow locus in Drosophila melanogaster. Dev Biol 48:336–343Google Scholar
  16. Nash WG, Yarkin RJ (1974) Genetic regulation and pattern formation: a study of the yellow locus in Drosophila melanogaster. Genet Res 24:19–26Google Scholar
  17. Pardue ML (1986) In situ hybridization to DNA of chromosomes and nuclei. In: Roberts DB (ed) Drosophila; a practical approach. IRL Press, Oxford, Washington DC, pp 111–138Google Scholar
  18. Parkhurst SM, Corces VG (1986) Interaction among the gypsy transposable element and the suppressor of Hairy wing loci in Drosophila melanogaster. Mol Cell Biol 6:47–53Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Pavel G. Georgiev
    • 1
    • 2
  • Tatiana I. Gerasimova
    • 1
  1. 1.N.I. Vavilov Institute of General GeneticsUSSR Academy of SciencesMoscowUSSR
  2. 2.W.A. Engelhardt Institute of Molecular BiologyUSSR Academy of SciencesMoscowUSSR

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