Journal of Comparative Physiology B

, Volume 162, Issue 4, pp 331–339 | Cite as

K+ current stimulation by Cl- in the midgut epithelium of tobacco hornworm (Manduca sexta)

I. Kinetics and effect of Cl--site-specific agents
  • Wolfgang Zeiske
  • Heike Schröder
  • Gabriele Alpert
Article

Summary

Goblet cells in the midgut epithelium of the tobacco hornworm (Manduca sexta larva, 5th instar) actively secrete K+. This can be measured as short-circuit current (Isc) when the tissue is mounted in an Ussing chamber and bathed in K+-rich standard saline containing 32 mmol K+ · l-1. Isc depends strictly on basolateral (i.e. haemolymph side) K+ and is therefore termed K+ current, IK. Basolateral, but not apical, chloride, bromide and iodide stimulate IK when compared to the baseline current recorded with gluconate-, nitrate- or thiocyanate-containing salines. So-called “Cl--specific” transport inhibitors (frusemide, 9-anthracene carboxylic acid, diphenylamine carboxylic acid and 4,4′-diisothiocyanato-stilbene-2,2′-disulphonic acid) reduce IK when added to the basolateral bath, whether Cl- or gluconate is the principal ambient anion. Cl- stimulates IK according to saturation kinetics. The Michaelis-Menten-type, K+ concentration-dependent, saturation of IK is altered in a highly specific manner when gluconate is replaced by Cl-: maximal K+ current, as well as the apparent Michaelis constant, are increased by a factor of 4. Since IK develops in these conditions exclusively via basolateral, Ba2+-blockable K+ channels, these results can be understood if it is assumed that haemolymph Cl- interferes with the K+ channel by simultaneously lowering the binding affinity for K+ ions and increasing their subsequent transfer rate across the basolateral goblet cell membrane.

Key words

K+ current Cl- stimulation Cl- blockers Midgut Manduca sexta larva 

Abbreviations

9-AC

9-anthracene carboxylic acid

DPC

diphenylamine carboxylic acid

DIDS

4,4′-diisothiocyanato-stilbene-2,2′-disulphonic acid

DMSO

dimethylsulphoxide

G

conductance [cellular (c), shunt (sh)]

Gt

transepithelial conductance

GK

K+ conductance

GCl

Cl- conductance

GNa

Na+ conductance

xG

conductance in absence of Cl-

GNa, KCl

transepithelial conductance with Cl- saline

GNa, Kglu

transepithelial conductance with gluconate saline

IK(max)

maximal K+ current

Isc

short-circuit current

K

Michaelis constant for saturating Cl- stimulation (index Cl) or K+ current saturation (index m)

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Wolfgang Zeiske
    • 1
  • Heike Schröder
    • 1
  • Gabriele Alpert
    • 1
  1. 1.Institut für Tierphysiologie und Angewandte Zoologie der Freien Universität BerlinBerlin 41FRG

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