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Molecular and General Genetics MGG

, Volume 219, Issue 3, pp 480–485 | Cite as

Identification and nucleotide sequence of the delta-lysin gene, hld, adjacent to the accessory gene regulator (agr) of Staphylococcus aureus

  • Lars Janzon
  • Sven Löfdahl
  • Staffan Arvidson
Article

Summary

A Tn551 insertional mutation in the accessory gene regulator (agr) locus of the Staphylococcus aureus chromosome resulted in the decreased production of at least seven extracellular toxins and enzymes and a simultaneous increase in the production of protein A and coagulase (Recsei et al. 1986). Adjacent to this locus we have now identified another gene, hld, transcribed into a 0.5 kb RNA which codes for the staphylococcal delta-lysin. The expression of hld, was totally repressed in a strain carrying the agr insertional mutation. Hybridization with strand-specific probes and primer extension analysis revealed that hld, and agr are transcribed in opposite directions, starting 188 nucleotides apart. The hld, gene is mainly expressed during the post-exponential growth phase and is totally repressed during early exponential growth. Determination of hld, mRNA half-life in different growth phases indicated that this regulation is at the level of transcription.

Key words

Gene regulation Transcriptional control DNA sequence Delta-lysin Staphylococcus aureus 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Lars Janzon
    • 1
  • Sven Löfdahl
    • 2
  • Staffan Arvidson
    • 1
  1. 1.Department of BacteriologyKarolinska InstitutetStockholmSweden
  2. 2.Department of BacteriologyNational Bacteriological LaboratoriesStockholmSweden

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