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Molecular and General Genetics MGG

, Volume 221, Issue 3, pp 459–465 | Cite as

Genetic and fine structure analysis of unc-26(IV) and adjacent regions in Caenorhabditis elegans

  • David L. Charest
  • Denise V. Clark
  • Michael E. Green
  • David L. Baillie
Article

Summary

The genetic organization of unc-26(IV) and adjacent regions was studied in Caenorhabditis elegans. We constructed a fine structure genetic map of unc-26(IV), a gene that affects locomotion and pharyngeal muscle movement but not muscle structure. Eleven alleles were positioned relative to each other recombinationally and were classified according to phenotypic severity. The unc-26 gene spans at least 0.026 map units, which is exceptionally large for a C. elegans gene. All but one allele, e205, are amorphic alleles. Interestingly, e205 is hypomorphic but also suppressible by the amber suppressor sup-7. Nineteen lethal mutations in the unc-26 region were isolated and characterized. The unc-26 region is subdivided into four zones by five deficiency breakpoints. These mutations fall into 15 complementation groups. The stages of development affected by these mutations were determined.

Key words

Caenorhabditis elegans unc-26 Intragenic mapping Lethal mutations 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • David L. Charest
    • 1
  • Denise V. Clark
    • 1
  • Michael E. Green
    • 1
  • David L. Baillie
    • 1
  1. 1.Institute of Molecular Biology and Biochemistry, Department of Biological SciencesSimon Fraser UniversityBurnabyCanada

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