Applied Microbiology and Biotechnology

, Volume 31, Issue 3, pp 228–233 | Cite as

Studies on immobilization of the thermophilic bacterium Thermus aquaticus YT-1 by entrapment in various matrices

  • Pawinee Kanasawud
  • Sigridur Hjörleifsdottir
  • Olle Holst
  • Bo Mattiasson
Biotechnology
Received:
Accepted:

Summary

Immobilization of the thermophilic bacterium Thermus aquaticus YT-1 has been studied using various entrapment techniques. Alginate, κ-carrageenan, agar, agarose and polyacrylamide were tested as supports during operation at 65°C at which the cells are known to produce protease when grown free in solution. Alginate showed toxic effects and no viability was observed after entrapment in Ca alginate or even after exposure of free living cells to sodium alginate. Polyacrylamide was observed to be the best support. Protease activity was closely related to the appearance of free cells in the medium.

Keywords

Sodium Agar Agarose Immobilization Living Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Adlercreutz P, Holst O, Mattiasson B (1985) Characterization of Gluconobacter oxydans immobilized in calcium alginate. Appl Microbiol Biotechnol 22:1–7Google Scholar
  2. Alfredsson GA, Kristjansson JK, Hjörleifsdottir S, Stetter KO (1988) Rhodothermus marinus gen, nov., sp. nov., a thermophilic halophilic bacterium from submarine hot springs in Iceland. J Gen Microbiol 134:299–306Google Scholar
  3. Brock T, Freeze H (1969) Thermus aquaticus gen. n. and sp. n., a non-sporulating extreme thermophile. J Bacteriol 98:289–297Google Scholar
  4. Brodelius P, Vandamme EJ (1987) Immobilized cell systems. In: Kennedy JF (ed) Biotechnology, vol 7a. VCH Publishers, Weinheim, pp 405–464Google Scholar
  5. Cheetham PSJ (1979) Physical studies on the mechanical stability of columns of calcium alginate pellets containing entrapped microbial cells. Enzyme Microb Technol 1:183–188Google Scholar
  6. Cheetham PSJ (1980) Developments in immobilized plants cells and their applications. In: Wiseman A (ed) Topics in enzyme and fermentation technology, vol 4. Ellis Horwood, Chichester, pp 189–238Google Scholar
  7. Degryse E, Glansdorff N, Piérard A (1978) A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol 117:189–196Google Scholar
  8. Hahn-Hägerdal B, Larsson M, Mattiasson B (1982) Shift in metabolism towards ethanol production in Saccharomyces cerevisiae using alteration of the physical-chemical environment. Biotechnol Bioeng Symp 12:199–202Google Scholar
  9. Klein J, Eng H (1979) Immobilized whole cells. Physical characterization. The measurement of abrasion. Dechema Monogr 84:292–299Google Scholar
  10. Klein J, Washausen P (1979) Immobilized whole cells. Physical characterization. Pressure stability (compression experiment). Dechema Monogr 84:277–284Google Scholar
  11. Klein J, Stock J, Vorlop KD (1983) Pore size and properties of spherical Ca-alginate biocatalysts. Eur J Appl Microbiol Biotechnol 18:86–91Google Scholar
  12. Kokubu T, Karube I, Suzuki S (1981) Protease production by immobilized mycelia of Streptomyces fradiae. Biotechnol Bioeng 23:29–39Google Scholar
  13. Matsuzawa H, Hamoaki M, Ohta T (1983) Production of thermophilic extracellular protease (aqualysin I and II) by Thermus aquaticus YT-1, an extreme thermophile. Agric Biol Chem 47:25–28Google Scholar
  14. Mattiasson B (1983) Immobilization methods. In: Mattiasson B (ed) Immobilized cells and organelles, vol 1. CRC Press, Boca Raton, pp 3–25Google Scholar
  15. Millet J (1970) Characterization of proteinases excreted by Bacillus subtilis marburg strain during sporulation. J Appl Bacteriol 33:207–219Google Scholar
  16. Nilsson K, Birnbaum S, Flygare S, Linse L, Schröder U, Jeppsson U, Larsson P-O, Mosbach K, Brodelius P (1983) A general method for the immobilization of cells with preserved viability. Eur J Appl Microbiol Biotechnol 17:319–326Google Scholar
  17. Sonnleitner B, Fiechter A (1986) Application of immobilized cells of Thermoanaerobium brockii for stereoselective reductions of oxo-acid esters. Appl Microbiol Biotechnol 23:424–429Google Scholar
  18. Stellwagen E (1984) Strategies for increasing the stability of enzymes. Ann NY Acad Sci 434:1–6Google Scholar
  19. Vorlop KD, Klein J (1983) New developments in the field of cell immobilization—formation of biocatalysts by ionotropic gelation. In: Lafferty RM (ed) Enzyme technology, 3rd Rotenburg Fermentation Symposium. Springer, Berlin Heidelberg New York, pp 219–235Google Scholar
  20. Vuillemard J-C, Terré S, Benoit S, Amiot J (1988) Protease production by immobilized growing cells of Serratia marcescens and Myxococcus xanthus in calcium alginate gel beads. Appl Microbiol Biotechnol 27:423–431Google Scholar
  21. Younes G, Nicaud J-M, Guespin-Michel J (1984) Enhancement of extracellular enzymatic activities produced by immobilized growing cells of Myxococcus xanthus. Appl Microbiol Biotechnol 19:67–69Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Pawinee Kanasawud
    • 1
  • Sigridur Hjörleifsdottir
    • 1
  • Olle Holst
    • 1
  • Bo Mattiasson
    • 1
  1. 1.Department of Biotechnology, Chemical CenterUniversity of LundLundSweden

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