Applied Microbiology and Biotechnology

, Volume 29, Issue 1, pp 5–10 | Cite as

Xanthan gum production by altered pathogenicity variants of Xanthomonas campestris

  • Maria Eugenia Ramírez
  • Leopoldo Fucikovsky
  • Federico García-Jiménez
  • Rodolfo Quintero
  • Enrique Galindo


Several morphologically different isolates of Xanthomonas campestris pv. campestris were obtained by treatment with N-methyl-N′-nitro-N-nitrosoguanidine. These variants were used to infect Brassica plants where several degrees of virulence were found. The strains were cultured in order to produce polysaccharide, which was recovered by precipitation and subjected to physical and chemical characterization. A relationship was noted between virulence and parameters such as the final viscosity of the culture, the viscosifying capacity of the polymer and the amount of acetyl substituents in the gum. Infrared spectral analysis revealed that intramolecular interaction of gum constituents could play a significant role in virulence. It is suggested that the degree of virulence could be used as a criterion for selecting and isolating producers of high quality xanthan gum.


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Maria Eugenia Ramírez
    • 1
  • Leopoldo Fucikovsky
    • 2
  • Federico García-Jiménez
    • 3
  • Rodolfo Quintero
    • 1
  • Enrique Galindo
    • 1
  1. 1.Centro de Investigación sobre Ingenieria Genética y BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavacaMéxico
  2. 2.Colegio de PosgraduadosChapingoMéxico
  3. 3.Instituto de QuímicaUniversidad Nacional Autónoma de MéxicoMéxicoMexico

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