Cancer Chemotherapy and Pharmacology

, Volume 15, Issue 2, pp 101–104 | Cite as

Kinetics of 7-hydroxy-methotrexate after high-dose methotrexate therapy

  • R. Erttmann
  • S. Bielack
  • G. Landbeck
Original Articles 7-Hydroxy-Methotrexate, Kinetics in Man, High-Dose MTX Therapy

Summary

Kinetics of 7-hydroxy-methotrexate (7-OH-MTX) excretion after high-dose methotrexate (MTX) (12 g/m2) therapy were monitored in 93 consecutive drug cycles of 19 adolescent patients with osteosarcoma. A reversed-phase HPLC method was used. Serum elimination was found to be monophasic with a mean half-life of 5.5 h. Shortly after the 4-h MTX infusion period 7-OH-MTX levels exceeded those of the parent compound. By 12 h after MTX infusion 7-OH-MTX levels were 16.5 times higher than those of MTX itself.

Autostimulation of MTX metabolism leading to enhanced 7-OH-MTX production after repeated drug cycles was not observed. The production of 7-OH-MTX decreased significantly from the first to the last high-dose MTX cycle of the adjuvant chemotherapy protocol.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. Erttmann
    • 1
  • S. Bielack
    • 1
  • G. Landbeck
    • 1
  1. 1.Department of Hematology and Oncology Children's HospitalUniversity of HamburgHamburg 20Federal Republic of Germany

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