Cancer Chemotherapy and Pharmacology

, Volume 10, Issue 1, pp 40–42 | Cite as

Binding of vinblastine to recrystallized human α1-acid glycoprotein

  • W. H. Steele
  • D. J. Haughton
  • H. E. Barber
Original Articles Vinblastine Acid Glycoprotein Protein Binding
Received:
Accepted:

Summary

The binding of vinblastine (VLB) to recrystallized human α1-acid glycoprotein (α1-AGP) dissolved in phosphate-buffered saline (pH 7.4) was determined at different drug concentrations using the technique of continuous ultrafiltration. Vinblastine was extremely highly bound (>99.0%) at a drug concentration of 4.0 μmol·l-1, dropping to under 60% at 65.0 μmol·l-1. Binding was best described by a two-class model with higher-(9.4 μM-1) and lower-(0.1 μM-1) affinity sites but with a similar number of binding sites (1.5 as against 1.1 lower-affinity sites). These results strongly suggest that α1-AGP would be a major binding protein for VLB in plasma.

Keywords

Cancer Research Drug Concentration Vinblastine Major Binding Major Binding Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • W. H. Steele
    • 1
  • D. J. Haughton
    • 2
  • H. E. Barber
    • 2
  1. 1.Department of Therapeutics & Clinical PharmacologyUniversity Medical BuildingsAberdeenGreat Britain
  2. 2.Department of PharmacologyUniversity Medical BuildingsAberdeenGreat Britain

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