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Cancer Chemotherapy and Pharmacology

, Volume 13, Issue 2, pp 145–147 | Cite as

Expression of cell surface P-glycoprotein by an Adriamycin-resistant murine fibrosarcoma

  • Raffaella Giavazzi
  • Norbert Kartner
  • Ian R. Hart
Short Communication P-Glycoprotein, Adriamycin Resistance, Murine Fibrosarcoma

Summary

Analysis of the cell membrane of Adriamycin (doxorubicin)-resistant UV-2237 ADMR murine fibrosarcoma cells revealed a 170,000-dalton component that is not found in the drug-sensitive parent or revertant cells. Immunoblot (Western blot) analysis showed that this component is similar to the 170,000-dalton P-glycoprotein found on the surface of Chinese hamster ovary cells that exhibit multidrug resistance. Thus, multidrug resistance and P-glycoprotein expression apparently can occur in a wide variety of cells, including the metastatic murine solid tumor cell line described here.

Keywords

Western Blot Doxorubicin Solid Tumor Tumor Cell Line Multidrug Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Raffaella Giavazzi
    • 1
  • Norbert Kartner
    • 2
  • Ian R. Hart
    • 1
  1. 1.Cancer Metastasis and Treatment Laboratory, LBI-Basic Research ProgramNCI-Frederick Cancer Research FacilityFrederickUSA
  2. 2.Ontario Cancer Institute, Princess Margaret Hospital, and Department of Medical BiophysicsUniversity of TorontoTorontoCanada

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