Applied Microbiology and Biotechnology

, Volume 26, Issue 4, pp 347–351 | Cite as

Characterization and production of a new extracellular polymer from Pseudomonas sp. GSP-910

  • Angeles Manresa
  • Maria Josè Espuny
  • Jesus Guinea
  • Francisco Comelles
Biotechnology
Received:
Revised:

Summary

A new strain, Pseudomonas sp. GSP-910 has been isolated from soil and has been found to produce large quantities of an extracellular, highly viscous polysaccharide in a simple salt medium. Good polymer production (6.16 g·l-1) occurs on a sucrose-containing medium (2%) at high phosphate concentration (80 mM·l-1) and 0.5 g·l-1 of nitrogen source NH4Cl. The relative proportions of sugars in the polymer are: glucuronic acid 8.8%, glucose 28.07%, galactose 56.8%, and it is partially acetylated (6.32%). The isolated polymer exhibits higher viscosity at dilute concentrations than xanthan gum and it is stable at different temperatures, over a wide range of pH and in the presence of monovalent salt. In the presence of divalent cation (CaCl2 0.5%), 910-gum in aqueous solution (1%) solidifies to a resilient gel.

Keywords

Polysaccharide Pseudomonas Galactose Nitrogen Source Relative Proportion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Angeles Manresa
    • 1
  • Maria Josè Espuny
    • 1
  • Jesus Guinea
    • 1
  • Francisco Comelles
    • 2
  1. 1.Departmento de Microbiologìa, Facultad de FarmaciaUniversidad de BarcelonaBarcelonaSpain
  2. 2.Instituto de Tecnologìa Quimìca y Textil(CSIC)BarcelonaSpain

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